I8638
Anti-Integrin β1 antibody, Mouse monoclonal
clone W1B10, purified from hybridoma cell culture
Sinonimo/i:
Anti-CD29
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About This Item
Prodotti consigliati
Origine biologica
mouse
Coniugato
unconjugated
Forma dell’anticorpo
purified from hybridoma cell culture
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
W1B10, monoclonal
Forma fisica
buffered aqueous solution
PM
antigen 100-140 kDa
contiene
15 mM sodium azide
Reattività contro le specie
chicken
tecniche
immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: 1:200 using denatured, non-reduced extract of cultured fibroblasts from chicken embryo lower leg
Isotipo
IgG1
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
chicken ... ITGB1(374058)
Descrizione generale
Monoclonal anti-β-1-Chicken Integrin (mouse IgG1 isotype) is derived from the W1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified chicken integrin from fibroblasts of 11 days old chicken embryos. Chicken integrin is a heterodimer consisting of the common β-1 subunit and two α subunits (α-5 or α-3). The β-1 subunit of chicken integrin is a 803-amino acid transmembrane glycoprotein consisting of a large extracellular domain and a short cytoplasmic domain.
Specificità
The antibody recognizes an epitope on the extracellular side of the avian integrin complex, and inhibits adhesion of embryo and tendon fibroblasts as well as skeletal myoblasts to both laminin and fibronectin. Under reducing and denaturing conditions using extracts of chicken embryo muscle cells, the antibody precipitates the β1 class integrin heterodimer as a broad band of ~140 kDa. In the absence of reducing agents, the integrin β1 subunit appears as bands at 100 kDa (immature form), 100 kDa (mature form), and 140 kDa (noncovalently associated α-subunits). The antibody may also be used for bioactivity studies in adhesion assays.
Immunogeno
chicken integrin.1
Applicazioni
Anti-Integrin β1 antibody, Mouse monoclonal has been used in western blot, fluorescent activated cell sorter (FACS) analysis.
Azioni biochim/fisiol
Integrin binding to extracellular matrix (ECM) ligands regulates cell adhesion, shape, motility, intracellular signalling and gene expression. The monoclonal anti-b-1 chicken integrin antibody (clone W1B10) has been used to inhibit chicken muscle cell adhesion in cell cultures.
Stato fisico
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin with 15 mM sodium azide.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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