MAB1997
Anti-Integrin β1 Antibody, clone MB1.2
clone MB1.2, Chemicon®, from rat
Sinonimo/i:
CD29 antigen, Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, fibronectin receptor beta subunit, integrin VLA-4 beta subunit, integrin beta 1, integrin beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
rat
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
MB1.2, monoclonal
Reattività contro le specie
mouse, human
Reattività contro le specie (prevista in base all’omologia)
rat
Confezionamento
antibody small pack of 25 μg
Produttore/marchio commerciale
Chemicon®
tecniche
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG2κ
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... ITGB1(3688)
Descrizione generale
Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.
Specificità
Reacts with β1 subunit of VLA (β1) integrins
Immunogeno
Mouse Integrin beta-1, as per (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132). Mouse Integrin beta-1, as per (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132).
Applicazioni
Anti-Integrin β1 Antibody, clone MB1.2 is an antibody against Integrin β1 for use in FC, IH, IP & WB.
Immunoprecipitation:
A previous lot of this anitbody was used to immunoprecipitate VLA (β1) integrins from lysate of 106 cell equivalent: 2 μg.
Flow cytometry:
A 10 μg/mL concentration of a previous lot was used in FC.
Immunohistochemistry in frozen tissue sections:
20 μg/mL from a previous lot was used. As a result of the wide tissue distribution of VLA (β1), binding of MAB1997 to diverse cell types is commonly observed.
Does not block VLA integrin mediated cell adhesion to matrix proteins. Activation of VLA (β1) integrin signaling properties by MAB1997 has not been characterized.
Western blot:
10 µg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples.
Optimal working dilutions must be determined by end user.
A previous lot of this anitbody was used to immunoprecipitate VLA (β1) integrins from lysate of 106 cell equivalent: 2 μg.
Flow cytometry:
A 10 μg/mL concentration of a previous lot was used in FC.
Immunohistochemistry in frozen tissue sections:
20 μg/mL from a previous lot was used. As a result of the wide tissue distribution of VLA (β1), binding of MAB1997 to diverse cell types is commonly observed.
Does not block VLA integrin mediated cell adhesion to matrix proteins. Activation of VLA (β1) integrin signaling properties by MAB1997 has not been characterized.
Western blot:
10 µg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples.
Optimal working dilutions must be determined by end user.
Qualità
Routinely evaluated by Western Blot on C2C12 lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected integrin β1 on 10 μg of C2C12 lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected integrin β1 on 10 μg of C2C12 lysate.
Descrizione del bersaglio
~88 kDa (non-glycosylated) and ~130 kDa ( glycosylated).
Linkage
Replaces: 04-1109
Stato fisico
Format: Purified
Purified rat monoclonal IgG2κ liquid in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% NaN3
Risultati analitici
Control
Human OVCAR5 cell line whole cell lysate
C2C12 lysate.
Human OVCAR5 cell line whole cell lysate
C2C12 lysate.
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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