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D8130

Sigma-Aldrich

5,5′-ditiobis(acido 2-nitrobenzoico)

≥98%, BioReagent, suitable for determination of sulfhydryl groups

Sinonimo/i:

3-carbossi-4-nitrofenil disolfuro, Acido 6,6′-dinitro-3,3′-ditiodibenzoico, Bis(3-carbossi-4-nitrofenil) disolfuro, DTNB, Reagente di Ellman

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500 MG
CHF 30.10
1 G
CHF 54.20
5 G
CHF 132.00
10 G
CHF 244.00
25 G
CHF 496.00
100 G
CHF 1’970.00

CHF 30.10

Spedizione prevista il21 marzo 2025

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Cambia visualizzazione
500 MG
CHF 30.10
1 G
CHF 54.20
5 G
CHF 132.00
10 G
CHF 244.00
25 G
CHF 496.00
100 G
CHF 1’970.00

About This Item

Formula condensata:
[-SC6H3(NO2)CO2H]2
Numero CAS:
69-78-3
Peso molecolare:
396.35
Beilstein:
1896821
Numero CE:
200-714-4
Numero MDL:
MFCD00007140
Codice UNSPSC:
12352106
ID PubChem:
24894189
NACRES:
NA.26

CHF 30.10

Spedizione prevista il21 marzo 2025

Richiedi un ordine bulk

Nome Commerciale

BioReagent

Livello qualitativo

200

Saggio

≥98%

Stato

powder

Punto di fusione

240-245 °C (dec.) (lit.)

Compatibilità

suitable for determination of sulfhydryl groups

Stringa SMILE

OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O

InChI

1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)
KIUMMUBSPKGMOY-UHFFFAOYSA-N

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Descrizione generale

Il 5,5′-ditiobis(2-acido nitrobenzoico) è detto anche DTNB o reagente di Ellman.[1] È un reagente solubile in acqua che interagisce con i gruppi tiolici e può essere utilizzato comodamente a pH neutro.[2] L′anione tiolato (RS-) reagisce con il reagente di Ellman (DTNB2-) per produrre l′anione 2-nitro-5-tiobenzoato (TNB2-) e un disolfuro misto (R-S-TNB-). Il TNB2- ha un elevato coefficiente di assorbimento molare e assorbe a 412 nm. Questo assorbimento è indipendente dal pH, a pH> 7,3.[3]

Applicazioni

Il reagente di Ellman può essere utilizzato per il test dell′acetilcolinesterasi e per marcare specificamente i residui di cisteina.[2] È un reagente sensibile per misurare il contenuto di sulfidrile libero in proteine, peptidi e tessuti.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves

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Certificati d'analisi (COA)

Lot/Batch Number
WXBF2505V
WXBF3196V
WXBF3940V
0000340525
0000340535

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Christian K Riener et al.
Analytical and bioanalytical chemistry, 373(4-5), 266-276 (2002-07-12)
Since its introduction in 1959, Ellman's reagent (5,5'-dithio-bis(2-nitrobenzoic acid)) has been the favorite reagent for spectrophotometric measurement of protein sulfhydryls. Meanwhile however, evidence has accumulated that many protein sulfhydryls give an incomplete reaction with Ellman's reagent, even during prolonged assay
Reassessment of Ellman's reagent.
P W Riddles et al.
Methods in enzymology, 91, 49-60 (1983-01-01)
Venkaiah Betapudi et al.
Science translational medicine, 12(527) (2020-01-24)
Chemical warfare nerve agents are organophosphorus chemical compounds that induce cholinergic crisis, leaving little or no time for medical intervention to prevent death. The current chemical treatment regimen may prevent death but does not prevent postexposure complications such as brain
Ellman's reagent: 5,5'-dithiobis(2-nitrobenzoic acid)--a reexamination.
P W Riddles et al.
Analytical biochemistry, 94(1), 75-81 (1979-04-01)
María Elena Quintanilla et al.
Frontiers in behavioral neuroscience, 14, 122-122 (2020-08-28)
Chronic ethanol intake results in brain oxidative stress and neuroinflammation, which have been postulated to perpetuate alcohol intake and to induce alcohol relapse. The present study assessed the mechanisms involved in the inhibition of: (i) oxidative stress; (ii) neuroinflammation; and
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Protocolli

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. To dissolve DTNB which alcohol should use?

    1 answer

    1. This product is tested for solubility in Ethanol at 10 mg/mL. Solutions may also be prepared at 5mM in 100 mM sodium or potassium phosphate buffer, pH 7.2, containing 0.1 mM EDTA. Please see the link below to review the entire product datasheet for additional information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/414/707/d8130pis.pdf

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  4. what is the storage temperature for this chemical

    1 answer

    1. This product can be stored at room temperature.

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