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Merck
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H0290

Sigma-Aldrich

Hartman′s Fixative

histological tissue fixative

Sinonimo/i:

Davidson′s Fixative

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
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About This Item

Codice UNSPSC:
12171500
NACRES:
NA.47

Forma fisica

liquid

applicazioni

hematology
histology

Temperatura di conservazione

room temp

Categorie correlate

Descrizione generale

Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination.

Applicazioni

An overnight fixative used for the visualization of lymph nodes in radical dissection specimens. It is helpful in visualizing both both breast and colon specimens by turning lymph nodes white. Moore and Barr used a version of Hartman′s fixative for fixing skin biopsy specimens for study of fine nuclear details. Rat testes for immunohistochemical and morphological comparison were fixed using conventional and modified Hartman′s fixative for 24 hours, while eyes and testes for other analyses were fixed for 48 hours.

Avvertenze

Danger

Classi di pericolo

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Organi bersaglio

Central nervous system, Respiratory system

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

75.2 °F

Punto d’infiammabilità (°C)

24 °C

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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α-Amilasi Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein

Sigma-Aldrich

A0521

α-Amilasi

Ringers solution 1/4 strength tablets suitable for microbiology, NutriSelect® Basic

Millipore

96724

Ringers solution 1/4 strength tablets

Glutaraldeide Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative

Sigma-Aldrich

G5882

Glutaraldeide

Formaldeide ACS reagent, 37 wt. % in H2O, contains 10-15% Methanol as stabilizer (to prevent polymerization)

Sigma-Aldrich

252549

Formaldeide

John R Latendresse et al.
Toxicologic pathology, 30(4), 524-533 (2002-08-22)
Most recent revisions of regulatory guidelines for testing effects of chemicals on reproduction recommend Bouin's fluid (BF) or a "comparable fixative" instead of formalin to preserve the morphologic detail of testes for histopathological evaluation. However, picric acid in BF is
Fixation of testes and eyes using a modified Davidson's fluid: comparison with Bouin's fluid and conventional Davidson's fluid.
Latendresse J R, et al.
Toxicologic Pathology, 30(4), 524-533 (2002)
Dan Wang et al.
Biology of reproduction, 102(5), 1033-1044 (2020-02-11)
Fertilization is one of the fundamental biological processes, but so far, we still do not have a full understanding of the underlying molecular mechanism. We have identified a human acrosome protein, LY6/PLAUR domain containing 4 (LYPD4), expressed specifically in human
Generation and functional characterization of a conditional Pumilio2 null allele.
Lin K, et al.
Journal of biomedical research (2017)
The sex chromatin in human malignant tissues.
K L MOORE et al.
British journal of cancer, 11(3), 384-390 (1957-09-01)
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