Unfortunately, the solution stability of this product at -20°C is not determined internally. However, various sources suggest frozen aliquots stored at -20°C are stable for at least 3-4 months. It is strongly recommend to protect the solution from light.
H0135
Hydrocortisone
γ-irradiated, powder, BioXtra, suitable for cell culture
Sinonimo/i:
11β,17α,21-Trihydroxypregn-4-ene-3,20-dione, 17-Hydroxycorticosterone, 4-Pregnene-11β,17α,21-triol-3,20-dione, Cortisol, Kendall’s compound F, Reichstein’s substance M
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Cambia visualizzazione
1 MG
CHF 142.00
About This Item
Formula empirica (notazione di Hill):
C21H30O5
Numero CAS:
Peso molecolare:
362.46
Beilstein:
1354819
Numero CE:
Numero MDL:
Codice UNSPSC:
12352209
ID PubChem:
NACRES:
NA.77
Origine biologica:
synthetic (organic)
Stato:
powder
Saggio:
≥80% (HPLC)
Prodotti consigliati
Origine biologica
synthetic (organic)
Livello qualitativo
Sterilità
γ-irradiated
Nome Commerciale
BioXtra
Saggio
≥80% (HPLC)
Stato
powder
tecniche
cell culture | mammalian: suitable
Punto di fusione
211-214 °C (lit.)
Solubilità
ethanol: 1 mg/mL, clear
Condizioni di spedizione
ambient
Temperatura di conservazione
room temp
Stringa SMILE
[H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO
InChI
1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
JYGXADMDTFJGBT-VWUMJDOOSA-N
Informazioni sul gene
human ... ABCB1(5243) , CYP3A4(1576) , NR3C1(2908) , SERPINA6(866)
mouse ... Abcb1a(18671) , Abcb1b(18669) , Ifng(15978) , Nos2(18126)
rat ... Ar(24208) , Nr3c1(24413)
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Descrizione generale
Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.
Applicazioni
Hydrocortisone is used for the following applications:
- Used in myogenic induction and identification[1]
- Used in long-term stromal cultures and methylcellulose cultures (one of the components in the culture medium)[2]
- Used in adipogenic differentiation (one of the components in adipogenic medium)[3]
- Used in cell culture: The human mammary epithelial cell MCF-10A cells were maintained in DMEM/F12 and supplemented with hydrocortisone along with other components[4]
- The SUM149 cell line was established from a primary IBC and grown in FBS supplemented with Ham′s F12 medium containing insulin and hydrocortisone[5]
- Used in differentiation assays of melanoma spheroid cells[6]
Azioni biochim/fisiol
Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency.
Stato fisico
powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw
Ricostituzione
To prepare 50 μg/mL stock solution; add 1 mL absolute ethanol, gently swirl to dissolve, add 19 mL sterile medium while mixing.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Repr. 1A - STOT RE 2
Organi bersaglio
Adrenal gland,Bone marrow
Codice della classe di stoccaggio
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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How long is the 50 μg/mL stock solution (1 mL absolute ethanol + 19 mL sterile medium) stable when stored in frozen aliquots at -20°C?
1 answer-
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What are the recommended storage conditions for stock solutions of item H0135, Hydrocortisone?
1 answer-
The stock solutions can be stored at 4°C and are stable for up to 1 month.
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