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GE28-9893-34

Sigma-Aldrich

HiLoad® 26/600 Superdex® 75 pg

Cytiva 28-9893-34, column L × I.D. 60 cm × 26 mm, 34 μm avg. part. size

Sinonimo/i:

Size exclusion chromatography

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About This Item

Codice UNSPSC:
41115700
NACRES:
SB.52

Materiali

glass column
glass fiber (polypropylene reinforced)

PM

3000-70000 Da (globular proteins)

Confezionamento

pkg of 1 ea

Produttore/marchio commerciale

Cytiva 28-9893-34

Parametri

<13 mL sample volume
<2.6 mL/min flow rate
0.3 Mpa (42 psi)

Volume del letto

320 mL

Lungh. × diam. int. colonna

60 cm × 26 mm

Matrice

agarose dextran composite
dextran-agarose particle platform
polymeric

Gruppo funzionale matrice

dextran-agarose phase
dextran-agarose phase

Dimens. media delle particelle

34 μm

cleaning

1-14

working range

3-12

Tecnica di separazione

size exclusion (SEC)

Descrizione generale

HiLoad® 26/600 Superdex® 75 prep grade are prepacked 53 mL XK columns designed for high-resolution preparative gel filtration chromatography separating small proteins and polynucleotides.

Superdex® prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges, Mr < 10 000 (Superdex® 30 pg), Mr 3 000 to 70 000 (Superdex® 75 pg), and Mr 10 000 600 000 (Superdex® 200 pg). The medium combines high mechanical strength with high hydrophilicity, allowing high flow rates, and minimal nonspecific interactions.

Caratteristiche e vantaggi

  • High resolution with short run times and good recovery.
  • High capacity at high flow rates.
  • Separations can be scaled up to production levels.
  • Prepacked for convenience and reproducibility.
  • 1/16” fittings for convenient connections to AKTA design systems.

Compatibilità

All commonly used buffers, 1 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile
for high-resolution preparative gel filtration chromatography separating proteins and polynucleotides

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Note legali

HiLoad is a registered trademark of Cytiva
Superdex is a registered trademark of Cytiva

Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Understanding pressure drop in chromatography systems is crucial for optimal functionality across different parts of the system.

Protocolli

Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.

This page shows how to refold proteins using gel filtration and products from Cytiva.

Ensure chromatographic sample clarity to avoid column clogging, reduce washing needs, and prolong column life for purification.

Contenuto correlato

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

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