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GE17-5123-10

benzamidin Seph 4Fast Flow H-sub

Cytiva 17-5123-10, pack of 25 mL

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About This Item

Codice UNSPSC:
13111000
NACRES:
NA.56

ligand

p-aminobenzamidine

Confezionamento

pack of 25 mL

Produttore/marchio commerciale

Cytiva 17-5123-10

Condizioni di stoccaggio

(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)

Matrice

highly cross-linked 4% agarose

Diametro medio

90 μm

cleaning in place

1-9

working range

2-8

Temperatura di conservazione

2-8°C

Descrizione generale

HiTrap® Benzamidine FF (high sub) is an excellent tool for removal of proteolytic activity from a protein.

Applicazioni

Benzamidine Sepharose 4 Fast FLow (high sub) is an excellent tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as, zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates

Caratteristiche e vantaggi

  • Benzamidine Sepharose 4 Fast FLow (high sub) is based on highly cross-linked 4% Agarose enabling high fLow rates without losing binding capacity
  • p-aminobenzamidine (pABA) immobilized on Sepharose 4 Fast FLow matrix gives high productivity, and allows easy scale-up.
  • Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins
  • For convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pittogrammi

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Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.

This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.

Vedi tutto

Protocolli

Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

This page shows how to prepare samples for purification with affinity chromatography.

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