10602400001
Roche
Thrombin
from human plasma
Sinonimo/i:
coagulation factor Iia, thrombin
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About This Item
Prodotti consigliati
Origine biologica
human plasma
Livello qualitativo
Stato
lyophilized
Attività specifica
~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.)
PM
Mr ~33.6 kDa
Confezionamento
pkg of 20 U
Produttore/marchio commerciale
Roche
pH ottimale
8.2-9.0
Condizioni di spedizione
wet ice
Descrizione generale
Thrombin is a Na+ activated allosteric serine protease. It belongs to chymotrypsin family. Thrombin is made of two polypeptide chains of 36 (A chain) and 259 (B chain) residues that are covalently attached with a disulfide bond.
Thrombin is a coagulation factor IIa. It is a serine endopeptidase that hydrolyzes peptide and ester bonds specifically at the carboxylic side of Arg. The enzyme converts fibrinogen to fibrin. The product contains EDTA and additional stabilizing agents.
Applicazioni
Thrombin has been used in the expression and purification of recombinant RELM (resistin-like moleculefamily members. It has also been used in platelet spreading on fibrinogen and immunostaining.
Thrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets.
Azioni biochim/fisiol
Thrombin plays an important role in blood coagulation. It serve as a procoagulant factor while transforming fibrinogen into an insoluble fibrin clot. Thrombin can act as anticoagulant by the activation of protein C.
Stato fisico
Lyophilizate
Nota sulla preparazione
Storage conditions (working solution): -15 to -25°C
Inhibitors: DFP, TLCK, PMSF, benzamidine, α1-antitrypsin, α2-macroglobulin, antithrombin III heparin, hirudin, and APMSF
Inhibitors: DFP, TLCK, PMSF, benzamidine, α1-antitrypsin, α2-macroglobulin, antithrombin III heparin, hirudin, and APMSF
Ricostituzione
The reconstituted solution contains 20 mM EDTA.
Important note:
Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces!
Important note:
Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces!
Stoccaggio e stabilità
Store at 2 to 8 °C. (Store dry!)
Altre note
For life science research only. Not for use in diagnostic procedures.
Esclusione di responsabilità
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 4 Inhalation - STOT RE 2
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
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I clienti hanno visto anche
Thrombin: Physiology and Disease (2010)
W Bode et al.
Protein science : a publication of the Protein Society, 1(4), 426-471 (1992-04-01)
Thrombin is a multifunctional serine proteinase that plays a key role in coagulation while exhibiting several other key cellular bioregulatory functions. The X-ray crystal structure of human alpha-thrombin was determined in its complex with the specific thrombin inhibitor D-Phe-Pro-Arg chloromethylketone
Scott Kaatz et al.
American journal of hematology, 87 Suppl 1, S141-S145 (2012-04-05)
The new oral anticoagulants dabigatran, rivaroxaban and apixaban have advantages over warfarin which include no need for laboratory monitoring, less drug-drug interactions and less food-drug interactions. However, there is no established antidote for patients who are bleeding or require emergent
M W Mosesson
Journal of thrombosis and haemostasis : JTH, 3(8), 1894-1904 (2005-08-17)
Fibrinogen molecules are comprised of two sets of disulfide-bridged Aalpha-, Bbeta-, and gamma-chains. Each molecule contains two outer D domains connected to a central E domain by a coiled-coil segment. Fibrin is formed after thrombin cleavage of fibrinopeptide A (FPA)
Partially defective store operated calcium entry and Hem (ITAM) signaling in platelets of serotonin transporter deficient Mice.
Wolf K, et al.
PLoS ONE, 11(1), e0147664-e0147664 (2016)
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