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GE17-0969-01

IgG Sepharose™ 6 Fast Flow

Cytiva 17-0969-01, pack of 10 mL

Sinonimo/i:

IgG Column

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

ligand

human IgG

Confezionamento

pack of 10 mL

Produttore/marchio commerciale

Cytiva 17-0969-01

Condizioni di stoccaggio

(20% Ethanol, 50 mM K2PO4, 1mM MgCl2 pH 7.2 in 20% Ethanol)

Matrice

highly cross-linked 6% agarose

Diametro medio

90 μm

cleaning in place

3-12

working range

3-10

Capacità

~3 mg binding capacity(Protein A)

Temperatura di conservazione

2-8°C

Descrizione generale

lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose™ 6 Fast Flow matrix, with human IgG covalently coupled to it. Medium allows high flow rates to be used for rapid and convenient single step purification of protein.

lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose™ 6 Fast Flow matrix, with human IgG covalently coupled to it. The improved mechanical characteristics of this Fast Flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.

The mechanical characteristics of this fast flow medium allows high flow rates to be used for rapid and convenient single step purification of protein A fusion protein conjungates produced in prokaryotic expression systems.

Caratteristiche e vantaggi

  • IgG coupled to the highly cross-linked 6% agarose matrix Sepharose™ 6 Fast Flow, designed for the rapid purification of protein A and protein A fusion conjugates
  • Binds at least 2 mg protein A/mL medium
  • Suitable for tandem affinity purification (TAP) of protein complexes

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sepharose is a trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Performing a Separation with IgG Sepharose 6 Fast Flow

Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.

Characteristics of Dextrin Sepharose® High Performance Products

This page shows the characteristics of Dextrin Sepharose High Performance products from Cytiva.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolli

Purification of Protein A-Tagged Proteins

This page shows how to purify protein A-tagged proteins using Cytiva products.

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from Cytiva.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Contenuto correlato

Pull-down di proteine

Saggi di pull-down, reagenti e protocolli per lo studio delle interazioni proteina-proteina in vitro utilizzando pull-down di affinità o GST pull-down, purificazione per affinità in tandem (TAP) e metodi di co-immunoprecipitazione.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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