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GE17-0921-07

IMAC Sepharose 6 Fast Flow

Cytiva 17-0921-07, pack of 25 mL

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56
Prezzi e disponibilità al momento non sono disponibili

Confezionamento

pack of 25 mL

Produttore/marchio commerciale

Cytiva 17-0921-07

Matrice

6% cross-linked agarose

Dimensione particelle

45-165 μm

Diametro medio

90 μm

cleaning in place

2-14(Medium uncharged with metal ions.)

Intervallo di esercizio

3-12

Capacità

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

Compatibilità

suitable for bioprocess medium

Categorie correlate

Descrizione generale

IMAC Sepharose 6 Fast FLow is a BioProcess IMAC medium, designed for purifying proteins and peptides that have an affinity for metal ions, such as histidine-tagged proteins but also untagged recombinant or native proteins.

Applicazioni

IMAC Sepharose 6 Fast FLow is a member of the Cytiva range of IMAC chromatography media for capture and intermediate purification.


IMAC Sepharose 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with a novel chelating ligand immobilized to the base matrix. IMAC Sepharose 6 Fast FLow is supplied free of metal ions, allowing the user to charge it with the most appropriate metal ion for purification of a target Protein.


The coupling chemistry and the novel ligand is designed to give minimal ligand leakage while providing high capacity.


IMAC Sepharose 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, IMAC Sepharose 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support

Caratteristiche e vantaggi

  • Uncharged IMAC resin allowing possibility to charge with your metal ion of choice for optimized selectivity
  • Convenient purification of histidine-tagged Proteins, also when Ni2+ is not the best choice of metal ion
  • As BioProcess medium it fulfills industrial demands for security of supply, robust performance and regulatory support.
  • Available in the convenient and time-saving prepacked HiTrap® and HiPrep formats.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HiPrep is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Pittogrammi

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Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


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Certificati d'analisi (COA)

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Articoli

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

How to optimize purification of histidine-tagged proteins using Cytiva products.

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

Vedi tutto

Protocolli

This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using Cytiva products.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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