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G4510

Millipore

Glutathione−Agarose

lyophilized powder

Sinonimo/i:

Glutathione-Agarose Resin, GSH-agarose, S-linked glutathione agarose

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About This Item

Numero MDL:
MFCD00131200
Codice UNSPSC:
41106500
NACRES:
NA.56

Forma fisica

lyophilized powder

Livello qualitativo

200

Classi chimiche degli analiti

proteins (GST)

Grado di funzionalizzazione

≥10 μmol per mL gel

tecniche

immunoprecipitation (IP): suitable
protein purification: suitable

Matrice

4% cross-linked beaded agarose

Attivazione matrice

epoxy

Gruppi immobilizzati alla matrice

sulfur

Braccio spaziatore

12 atoms (10 carbon)

Temperatura di conservazione

−20°C

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Descrizione generale

Glutathione S-transferase (GST) is a protein with a molecular weight of 26 KDa that naturally occurs in eukaryotic cells. It belongs to the family of Phase II detoxification enzymes. Glutathione-Agarose consists of glutathione attached through its sulfur to epoxy-activated 4% cross-linked beaded agarose, resulting in a 12-atom (10-carbon) spacer. It is provided as a lyophilized powder stabilized with lactose.

Applicazioni

Glutathione-Agarose has been used:
  • to purify glutathione S-transferase (GST)-fused protein for generating polyclonal antibody M4P
  • to incubate supernatant fractions for the purification of full-length p53 (aa 1-393) and its DNA-binding domain (aa 94-288)
  • in affinity purification of the fusion protein

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

Azioni biochim/fisiol

Glutathione S-transferases (GSTs), a superfamily of multifunctional detoxification isoenzymes, have a significant role in cellular signaling. GSTs are emerging as promising therapeutic targets due to the overexpression of specific isozymes in various tumors and their potential involvement in the development of other diseases such as neurodegenerative diseases, multiple sclerosis, and asthma.

Caratteristiche e vantaggi

  • Offers rapid, mild, non-denaturing, and highly selective purification of glutathione-binding enzymes.
  • Has a shelf life of two years if stored properly desiccated at -20 °C.
  • Binding capacity: 5-10 mg glutathione S-transferase per mL resin

Quantità

One g powder swells to ≥10 ml of gel

Stato fisico

Lyophilized powder stabilized with lactose

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Sigma-Aldrich

G4251

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Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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