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G0924

Millipore

Glutathione High Capacity Magnetic Agarose Beads

(1:1 suspension in a 30% ethanol solution)

Sinonimo/i:

Magnetic agarose beads

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1 ML
CHF 101.00
5 X 1 ML
CHF 358.00

CHF 101.00

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Tutte le immagini(1)

Scegli un formato

Cambia visualizzazione
1 ML
CHF 101.00
5 X 1 ML
CHF 358.00

About This Item

Numero MDL:
MFCD00131200
Codice UNSPSC:
41106500
NACRES:
NA.56

CHF 101.00

Check Cart for Availability

Coniugato

magnetic beads

Livello qualitativo

200

Stato

(1:1 suspension in a 30% ethanol solution)

Classi chimiche degli analiti

proteins (GST)

tecniche

immunoprecipitation (IP): suitable
protein purification: suitable

Matrice

4% beaded magnetic agarose

Braccio spaziatore

12 atoms

Capacità

≥15 μmol/mL, agarose binding capacity

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

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Descrizione generale

Glutathione Magnetic Agarose Beads consist of a paramagnetic 4% beaded agarose. Glutathione is covalently attached through the sulfur to epoxy-activated agarose resulting in a 12 atom (10 carbon) spacer.

The Glutathione Magnetic Agarose Beads are useful for affinity capture, molecular pull-down, or immunoprecipitation (IP) of proteins containing glutathione binding sequences, such as native glutathione S-transferase (GST), glutathione peroxidase, and glyoxalase I, or GST-tagged recombinant fusion proteins from cell lysates or other biochemical solutions while exhibiting low non-specific binding of other proteins. Recombinant GST-tagged proteins, bound to the affinity resin are separated with the use of a magnet. The magnetic properties allow for very rapid processing, as well as aid in manipulations, such as repetitive washings and recovery of the desired protein. This leads to faster recovery, experimental reproducibility, and more accurate quantitation of the proteins of interest.

The matrix of the magnetic beads is a 4% beaded agarose with an average diameter of 50 μ and a diameter range of 20-75 μ. Paramagnetic iron is impregnated within the beads.

Applicazioni

Useful for affinity capture, molecular pull-down, or immunoprecipitation (IP) of proteins containing glutathione binding sequences, such as recombinant GST-tagged fusion proteins or native glutathione S-transferase, glutathione peroxidase and glyoxalase I from cell lysates and other biological samples. Can be used for purification in single tubes or for high throughput in 96-well multiwell plates. The magnetic properties of the beads aid in manipulations, such as repetitive washings and recovery of the protein. This leads to greater experimental reproducibility and more accurate quantitation of the proteins of interest.

Stato fisico

1:1 suspension in a 30% ethanol solution

Pittogrammi

Flame
GHS02

Avvertenze

Danger

Indicazioni di pericolo

H225

Classi di pericolo

Flam. Liq. 2

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

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Certificati d'analisi (COA)

Lot/Batch Number
0000408611
0000408611
0000401503
0000401503
0000360954

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Xueran Chen et al.
Theranostics, 10(3), 998-1015 (2020-01-16)
Rationale: Glioblastoma multiforme (GBM) almost invariably gain invasive phenotype with limited therapeutic strategy and ill-defined mechanism. By studying the aberrant expression landscape of gliomas, we find significant up-regulation of p-MAPK level in GBM and a potent independent prognostic marker for
Reinier F Prosée et al.
PLoS biology, 19(7), e3000968-e3000968 (2021-07-07)
Centromere protein A (CENP-A) is a histone H3 variant that defines centromeric chromatin and is essential for centromere function. In most eukaryotes, CENP-A-containing chromatin is epigenetically maintained, and centromere identity is inherited from one cell cycle to the next. In
Yong-Wei Nie et al.
Biochemical and biophysical research communications, 473(1), 249-254 (2016-03-24)
Sorting motifs are involved in the transport of diverse proteins. In the present study, we identified a hydrophobic peptide (WRPWRNFWWSIRVPWRRN) that was able to target enhanced green fluorescent protein- or DsRed2-enriched vesicular-like sub-compartments of the endoplasmic reticulum (ER). Analysis of
Yun Wei et al.
Journal of materials chemistry. B, 1(15), 2066-2071 (2013-04-21)
The imidazolium cation was chosen as protein selective affinity group and a kind of ionic liquid magnetic microspheres was developed by immobilizing imidazolium cations onto the surface of silica-coated magnetic microspheres to form imidazolium ionic liquid modified magnetic microspheres (Fe3O4@SiO2@IL).
April N Ilacqua et al.
BMC cell biology, 19(1), 13-13 (2018-07-18)
Copines are calcium-dependent phospholipid-binding proteins found in many eukaryotic organisms and are thought to be involved in signaling pathways that regulate a wide variety of cellular processes. Copines are characterized by having two C2 domains at the N-terminus accompanied by
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Contenuto correlato

Pull-down di proteine

Per studiare in vitro le interazioni proteina-proteina potete ricorrere a saggi di pull-down per affinità, o GST pull-down, purificazione per affinità in tandem (TAP) e co-immunoprecipitazione.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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