Passa al contenuto
Merck
Tutte le immagini(1)

Documenti fondamentali

Visualizza tutta la documentazione

G3907

Millipore

Glutathione−Agarose

set of 3 pre-packed columns (2.5 ml each), (1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

Sinonimo/i:

GSH-agarose, S-linked glutathione agarose

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali

Scegli un formato

Tutte le immagini(1)

Scegli un formato

Cambia visualizzazione

About This Item

Numero MDL:
MFCD00131200
Codice UNSPSC:
41106500
NACRES:
NA.56

Livello qualitativo

200

Stato

(1:1 suspension in a 0.5 M NaCl + 20% ethanol solution)

Classi chimiche degli analiti

proteins (GST)

Confezionamento

set of 3 pre-packed columns (2.5 ml each)

tecniche

immunoprecipitation (IP): suitable
protein purification: suitable

Matrice

cross-linked 4% beaded agarose

Temperatura di conservazione

2-8°C

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Descrizione generale

The resin in these columns consists of glutathione attached through the sulfur to epoxy activated, 4% cross-linked beaded agarose resulting in a 12 atom spacer. [1]

Applicazioni

Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of glutathione binding enzymes such as glutathione-S-transferase, glutathione peroxidase, and glyoxalase I.[2]
The product was used in the design, synthesis and biological evaluation of new tryptamine and tetrahydro-β-carboline-based selective inhibitors of CDK4.[3] It was also used to study Fascaplysin-inspired diindolyls as selective inhibitors of CDK4/cyclin D1.[4]
Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I.

Linkage

Suspension of Product No. G 4510

Stato fisico

1:1 suspension in a 0.5 M NaCl + 20% ethanol solutionl

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

180.1 °F

Punto d’infiammabilità (°C)

82.3 °C

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
SLBR3849V
016K7056
074K7025

Non trovi la versione di tuo interesse?

Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

Cerca un COA

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 4

1 of 4

GST Bulk Kit Cytiva 27-4570-01, pack of 1 kit

GE27-4570-01

GST Bulk Kit

Glutathione Sepharose™ High Performance Cytiva 17-5279-01, pack of 25 mL

GE17-5279-01

Glutathione Sepharose™ High Performance

PreScission Protease Cytiva 27-0843-01

GE27-0843-01

PreScission Protease

L-Glutathione reduced ≥98.0%

Sigma-Aldrich

G4251

L-Glutathione reduced

Paul R Jenkins et al.
Bioorganic & medicinal chemistry, 16(16), 7728-7739 (2008-07-25)
We present the design, synthesis and biological activity of a library of substituted (biphenylcarbonyl)-tryptamine and (biphenylcarbonyl)-tetrahydro-beta-carboline compounds related to the natural product fascaplysin, as novel inhibitors of CDK4/cyclin D1. We show all these molecules, prepared using the Suzuki-Miyaura reaction, being
Carine Aubry et al.
Organic & biomolecular chemistry, 4(5), 787-801 (2006-02-24)
We present the design, synthesis, and biological activity of three classes of tryptamine derivatives, which are non-planar analogues of the toxic anti-cancer agent fascaplysin. We show these compounds to be selective inhibitors of CDK4 over CDK2, the most active compound
Diane M Kanter et al.
Nucleic acids research, 39(7), 2580-2592 (2010-11-27)
Sld2 is essential for the initiation of DNA replication, but the mechanism underlying its role in replication is not fully understood. The S-phase cyclin dependent kinase (S-CDK) triggers the association of Sld2 with Dpb11, and a phosphomimetic mutation of Sld2
F Toribio et al.
Journal of chromatography. B, Biomedical applications, 684(1-2), 77-97 (1996-09-20)
The different preparative techniques and related analytical methods used for purification of glutathione peroxidase, glutathione transferase and glutathione reductase, described in papers published in the last ten years, have been reviewed in this article. Among the different purification techniques, chromatography
Dustin L Johnson et al.
Journal of bacteriology, 190(8), 2972-2980 (2008-02-19)
Chlamydophila pneumoniae is a gram-negative obligate intracellular bacterial pathogen that causes pneumonia and bronchitis and may contribute to atherosclerosis. The developmental cycle of C. pneumoniae includes a morphological transition from an infectious extracellular elementary body (EB) to a noninfectious intracellular
Visualizza tutti i documenti correlati

Contenuto correlato

Pull-down di proteine

Per studiare in vitro le interazioni proteina-proteina potete ricorrere a saggi di pull-down per affinità, o GST pull-down, purificazione per affinità in tandem (TAP) e co-immunoprecipitazione.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Questions

Reviews

No rating value

Active Filters

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.