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G4252

Sigma-Aldrich

Glyoxalase I from Saccharomyces cerevisiae

Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein

Sinonimo/i:

S-Lactoyl-glutathione methylglyoxal-lyase (isomerizing)

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About This Item

Numero CAS:
9033-12-9
Classificazione EC (Enzyme Commission):
4.4.1.5 (BRENDA, IUBMB)
Numero MDL:
MFCD00131225
Codice UNSPSC:
12352204
NACRES:
NA.54

Tipo

Grade IV

Forma fisica

buffered aqueous glycerol solution

Attività specifica

≥400 units/mg protein

Attività estranea

glyoxalase II ≤1%

Temperatura di conservazione

2-8°C

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Descrizione generale

Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme.

Applicazioni

Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels.

Azioni biochim/fisiol

Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner.

Definizione di unità

One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C.

Stato fisico

Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5

Risultati analitici

Protein determined by biuret.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificati d'analisi (COA)

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Xuming Jia et al.
PloS one, 7(5), e36610-e36610 (2012-05-19)
Methylglyoxal (MG) is a highly reactive metabolite physiologically presented in all biological systems. The effects of MG on diabetes and hypertension have been long recognized. In the present study, we investigated the potential role of MG in obesity, one of
On glyoxalase
H.D. Dakin & H.W. Dudley
The Journal of Biological Chemistry, 14, 423-431 (1913)
Megan Jack et al.
Translational research : the journal of laboratory and clinical medicine, 159(5), 355-365 (2012-04-17)
Diabetic neuropathy is the most common and debilitating complication of diabetes mellitus with more than half of all patients developing altered sensation as a result of damage to peripheral sensory neurons. Hyperglycemia results in altered nerve conduction velocities, loss of
Víctor Cubillos et al.
Marine environmental research, 119, 59-71 (2016-05-28)
The estuarine slipper limpet Crepipatella dilatata is a gastropod that can survive prolonged periods of low salinities (< 24 PSU) caused by tidal changes and/or prolonged periods of rain. During low salinity events, C. dilatata can isolate its body from the outside
The mechanism of action of glyoxalase.
E RACKER
The Journal of biological chemistry, 190(2), 685-696 (1951-06-01)
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