C9584

Carboxypeptidase B from porcine pancreas

lyophilized powder

• Sinonimo/i: Peptidyl-L-lysine(L-arginine) hydrolase, Protaminase
• Numero CAS: 9025-24-5
• Classificazione EC (Enzyme Commission): 3.4.17.2 (BRENDA, IUBMB)
• Numero CE: 232-788-9
• Numero MDL: MFCD00081476
• Codice UNSPSC: 12352204
• ID PubChem: 24893175
• NACRES: NA.54

Proprietà

• Origine biologica: Porcine pancreas
• Grado: Proteomics Grade
• Forma fisica: lyophilized powder
• Attività specifica: ≥125 units/mg protein
• PM: 34,000 Da± 600
• Purificato mediante: affinity chromatography
• Composizione: Protein, 40-70%
• Attività estranea: carboxypeptidase A ≤1%; chymotrypsin ≤0.1%; trypsin ≤5%
• Temperatura di conservazione: −20°C
• InChI: 1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38); TWURVFFNODFJBJ-UHFFFAOYSA-N

Descrizione

Applicazioni

Carboxypeptidase B has been used in a study to develop a non-invasive pregnancy assay for use in both captive and wild polar bears. Carboxypeptidase B has been used in a study that identified new potential biomarkers of acute pancreatitis.

The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.

Azioni biochim/fisiol

Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.

Confezionamento

Package size based on protein content

Definizione di unità

One unit will hydrolyze 1.0 μmole of hippuryl-L-arginine per min at pH 7.65 at 25 °C.

Stato fisico

Contains HEPES buffer salts, zinc chloride and carbohydrate

Nota sulla preparazione

Treated with protease inhibitor, AEBSF, to eliminate serine protease activity.

Risultati analitici

Protein determined by biuret.

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H315,H319,H334,H335
• Consigli di prudenza: P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves