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C3888

Carboxypeptidase Y from baker′s yeast (S. cerevisiae)

lyophilized powder, ≥50 units/mg protein

Sinonimo/i:

Peptidyl-L-amino acid Hydrolase, Serine Carboxypeptidase

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Cambia visualizzazione
A voi/SKUDisponibilitàPrezzo
1 mg
Per conoscere la disponibilità, visualizza il carrello
CHF 105.00
5 mg
Per conoscere la disponibilità, visualizza il carrello
CHF 343.00

Informazioni su questo articolo

Numero CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
Numero CE:
MDL number:
Specific activity:
≥50 units/mg protein

CHF 105.00


Per conoscere la disponibilità, visualizza il carrello

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grade

Proteomics Grade

form

lyophilized powder

specific activity

≥50 units/mg protein

mol wt

61 kDa

composition

Protein, ≥75% E1%/280

shipped in

wet ice

storage temp.

−20°C

Application

Carboxypeptidase Y has a broad specificity and is stable in urea. Hence, this enzyme is not like other carboxypeptidases and can be used for sequence analysis. Due to its amidase action, this enzyme might be applied to the sequence analysis of peptides having amidated COOH-terminal groups such as oxytocin and vasopressin.[1]

Biochem/physiol Actions

The glycoprotein has a molecular weight of about 61,000, has a nitrogen content of 12.74%. It is a single polypeptide chain of 442 residues with 16 residues of glucosamine in the carbohydrate moiety. Lysine is at the NH2 terminus and -Asp-Ser-Thr-Leu is the COOH-terminal sequence. The principal action of the enzyme is to remove COOH-terminal residues from polypeptide chains.[1] It is used as a vacuolar marker enzyme for studies on protein transport and localization.[2]

Packaging

Package size based on protein content

Physical form

Lyophilized powder containing citric acid.

Analysis Note

amidase and esterase activities may be present

Other Notes

One unit will hydrolyze 1.0 μmole of N-CBZ-Phe-Ala to N-CBZ-L-phenylalanine and L-alanine per min at pH 6.75 at 25°C, based on EM/230 = 191.5.

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Questo articolo
C9584C9658C9268
grade

Proteomics Grade

grade

Proteomics Grade

grade

-

grade

Proteomics Grade

specific activity

≥50 units/mg protein

specific activity

≥125 units/mg protein

specific activity

≥3 units/mg protein

specific activity

≥50 units/mg protein

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

ready-to-use solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

mol wt

61 kDa

mol wt

34,000 Da± 600

mol wt

homodimer ~90 kDa

mol wt

~35 kDa


pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Classe di stoccaggio

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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