Biotinylation reagent incorporating a spacer with a cleavable disulfide linkage. Typically coupled to primary amine in the pH range 6.5-8.5. Cleavage of disulfide requires reducing agent allowing mild recovery of biotinylated compound.
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Clinical and experimental immunology, 136(2), 365-372 (2004-04-17)
Hodgkin's lymphoma patients treated with an anti-CD25 Ricin toxin A-chain (RTA)-based Immunotoxin (RFT5.dgA) develop an immune response against the toxic moiety of the immunoconjugate. The anti-RTA antibody response of 15 patients showing different clinical features and receiving different total amounts
We have prepared several mechanism-based affinity-labeling agents for possible use in isolating N-acetylglucosaminidase, in which an N-acetylglucosamine is linked to an o-monofluoro- or difluoro-methyl phenoxy glycoside with or without a cleavable disulfide group in the tether to biotin.
A reagent for covalently attaching biotin to proteins via a cleavable connector arm.
C A Mouton et al.
Archives of biochemistry and biophysics, 218(1), 101-108 (1982-10-01)
Chemical probes of extended biological structures: synthesis and properties of the cleavable protein cross-linking reagent [35S]dithiobis(succinimidyl propionate).
A J Lomant et al.
Journal of molecular biology, 104(1), 243-261 (1976-06-14)
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