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B1267

Sigma-Aldrich

Biotin-maleimide

≥95% (TLC), powder

Sinonimo/i:

N-Biotinoyl-N′-(6-maleimidohexanoyl)hydrazide

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25 MG
CHF 220.00
100 MG
CHF 454.00

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25 MG
CHF 220.00
100 MG
CHF 454.00

About This Item

Formula empirica (notazione di Hill):
C20H29N5O5S
Numero CAS:
Peso molecolare:
451.54
Numero MDL:
Codice UNSPSC:
12352203
ID PubChem:
NACRES:
NA.46

CHF 220.00


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Saggio

≥95% (TLC)

Stato

powder

Solubilità

acetic acid: 20 mg/mL

Temperatura di conservazione

2-8°C

Stringa SMILE

O=C(CCCCCN1C(=O)C=CC1=O)NNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23

InChI

1S/C20H29N5O5S/c26-15(7-2-1-5-11-25-17(28)9-10-18(25)29)23-24-16(27)8-4-3-6-14-19-13(12-31-14)21-20(30)22-19/h9-10,13-14,19H,1-8,11-12H2,(H,23,26)(H,24,27)(H2,21,22,30)/t13-,14-,19-/m0/s1
VAPZWEFPQRDVHR-NJSLBKSFSA-N

Descrizione generale

Biotin-maleimide is a biotin specific thiol containing reagent used to biotinylate different proteins through artificially induced or natural sulphadryl group. It can be used as a universal, multipurpose, thiol-specific probe. Biotin-maleimide can also be used in dot blots to detect protein SH groups[1].

Applicazioni

Biotin-maleimide- 0.01 mg/ml in PBS containing 0.1% Tween 20, incubated for 1 h at room temperature can be used to detect deprotected cysteine thiols present on immobilized peptides[2].
Sulfhydryl-specific biotinylation reagent. Couples by highly efficient thioether linkage. Typically used to couple to intrinsic sulfhydryls, e.g. protein, and avoid modification of primary amine.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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A biotin-containing, thiol-specific reagent, 3-(N-maleimido-propionyl) biocytin (MPB), was synthesized and used to biotinylate various proteins via native or artificially induced sulfhydryl groups. In combination with appropriate avidin- or streptavidin-conjugated markers (i.e., fluorescent, enzyme-conjugated, electron-dense, etc.), MPB essentially constitutes a universal
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Questions

  1. What is the solubility of B1267 Biotin-maleimide in DMSO?

    1 answer
    1. The B1267 Biotin-maleimide is a white powder that is soluble in DMSO at 10mg/ml, resulting in a clear colorless solution. A stock solution of the product in DMSO at 20 mg/ml can be prepared without the need for heat. This solution can then be diluted 1:10 with an aqueous buffer system, such as PBS or other. Concentrations of 40-50 mg/ml can also be prepared, but heat, i.e. 40°C, is needed. For most applications, a stock of 20 mg/ml would be suitable.

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