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A9415
Actinomycin D
from Streptomyces sp., suitable for cell culture, ≥95%
Sinonimo/i:
Cosmegen, Meractinomycin, Actinomycin C1, Actinomycin IV, Dactinomycin
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About This Item
Formula empirica (notazione di Hill):
C62H86N12O16
Numero CAS:
Peso molecolare:
1255.42
Beilstein:
605235
Numero CE:
Numero MDL:
Codice UNSPSC:
51282001
ID PubChem:
NACRES:
NA.76
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Origine biologica
Streptomyces sp.
Livello qualitativo
Saggio
≥95%
Stato
powder
tecniche
cell culture | mammalian: suitable
Colore
red, powder
Punto di fusione
251-253
Solubilità
ethanol, DMSO: soluble (Stable in aqueous solutions at 2-8 °C.)
Spettro attività antibiotica
neoplastics
Modalità d’azione
DNA synthesis | interferes
Temperatura di conservazione
2-8°C
Stringa SMILE
CC(C)[C@H]1NC(=O)[C@@H](NC(=O)c2ccc(C)c3OC4=C(C)C(=O)C(N)=C(C(=O)N[C@H]5[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]6CCCN6C(=O)[C@H](NC5=O)C(C)C)C4=Nc23)[C@@H](C)OC(=O)[C@H](C(C)C)N(C)C(=O)CN(C)C(=O)[C@@H]7CCCN7C1=O
InChI
1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)/t33-,34-,36+,37+,42-,43-,44+,45+,48+,49+/m1/s1
RJURFGZVJUQBHK-IIXSONLDSA-N
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Categorie correlate
Descrizione generale
Chemical structure: peptide
Applicazioni
Actinomycin D is an antineoplastic antibiotic that inhibits cell proliferation with wide-ranging applications ranging from a selection agent in cell culture, use in studies of suppressing HIV-replication and programmed cell death of PC12 cells.. Recommended for us in cell culture applications at 1μg/mL.
Azioni biochim/fisiol
Actinomycin D inhibits the proliferation of cells by forming a stable complex with double-stranded DNA, inhibiting DNA-primed RNA synthesis and causing single-stranded breaks in DNA. It has been shown to be an inhibitor of the minus-strand transfer step in reverse transcriptase.
Avvertenza
This product is hygroscopic and light-sensitive. If stored protected from light and moisture at 2-8°C, it remains unchanged, by HPLC testing, for at least 15 months. Unused dilute solutions of Actinomycin D are extremely sensitive to light and tend to adsorb to plastic and glass and should be discarded. However, frozen aliquots of concentrated stock solution are stable for at least a month.
Nota sulla preparazione
Actinomycin D is sold as red, shiny crystals and is soluble in acetonitrilie or acetone at 10 mg/mL and in DMSO at a minimum of 1 mg/mL. Some references also show slight solubility in water of 0.5 mg/mL.
Altre note
Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Acute Tox. 2 Oral - Carc. 1B - Repr. 1B
Codice della classe di stoccaggio
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
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Certificati d'analisi (COA)
Lot/Batch Number
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Articoli
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Protocolli
WST-1 assay protocol for cell viability and cytotoxicity measurements with preparation instructions, applications, FAQs, and troubleshooting.
Protocollo del saggio di proliferazione e vitalità cellulare e citotossicità con MTT. Istruzioni per la preparazione dei reagenti per il saggio con MTT con esempi di applicazione.
Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.
MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.
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What is the Department of Transportation shipping information for this product?
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What is the working concentration for Actinomycin D (Product No. A9415)?
1 answer-
For cell culture applications, actinomycin D is used as a selection agent and is used in banding techniques to differentiate between different regions of chromosomes. Suggested working concentrations is 1 μg/mL.
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How do you recommend dissolving Product No. A9415 (Actinomycin D)?
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Stock solutions of this product may be prepared at 10 mg/mL in either ethanol (EtOH) or dimethyl sulfoxide (DMSO).
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How long are stock solutions of Actinomycin D (Product No. A9415) stable?
1 answer-
Stock solutions of this product in DMSO or ethanol should be stored in foil-wrapped vials at -20 °C. In DMSO, solution can be stored for up to 1 month at -20 °C. Do not store in glass vials.
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