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A6751

Sigma-Aldrich

N-Acetyl-L-tyrosine ethyl ester monohydrate

≥98%, suitable for ligand binding assays

Sinonimo/i:

ATEE

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About This Item

Formula empirica (notazione di Hill):
C13H17NO4 · H2O
Numero CAS:
36546-50-6
Peso molecolare:
269.29
Beilstein:
2217900
Numero CE:
212-663-5
Numero MDL:
MFCD00002389
Codice UNSPSC:
12352209
eCl@ss:
32160406
ID PubChem:
24891134
NACRES:
NA.26

product name

N-Acetyl-L-tyrosine ethyl ester monohydrate,

Saggio

≥98%

Forma fisica

powder

tecniche

ligand binding assay: suitable

Colore

white

Temperatura di conservazione

−20°C

Stringa SMILE

CCOC(=O)[C@H](Cc1ccc(O)cc1)NC(C)=O

InChI

1S/C13H17NO4/c1-3-18-13(17)12(14-9(2)15)8-10-4-6-11(16)7-5-10/h4-7,12,16H,3,8H2,1-2H3,(H,14,15)/t12-/m0/s1
SKAWDTAMLOJQNK-LBPRGKRZSA-N

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Applicazioni

N-Acetyl-L-tyrosine ethyl ester monohydrate has been used:
  • as a substrate in transesterification reactions with 1 M propan-1-ol catalyzed by Carlsberg protease protein-coated microcrystals (PCMC)
  • to test the performance of subtilisin Carlsberg protein-coated microcrystals (PCMC)
  • as a substrate for cross-linked crystals (CLECs) of subtilisin activity screening

Azioni biochim/fisiol

N-Acetyl-L-tyrosine ethyl ester is an N-terminal and C-terminal protected L-tyrosine that is used in crosslinking studies and as a substrate for the detection, differentiation and/or characterization various proteases and esterases.

Substrati

Substrate for chymotrypsin

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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USP

USP

1134007

Chymotrypsin

Operational stability of subtilisin CLECs in organic solvents in repeated batch and in continuous operation
Fernandes JFA, et al.
Biochemical Engineering Journal, 24(1) (24)
Stability of protein-coated microcrystals in organic solvents
Kreiner M, et al.
Journal of Molecular Catalysis. B, Enzymatic, 33(3-6) (33)
Alicja Janik et al.
Acta crystallographica. Section C, Crystal structure communications, 63(Pt 10), o572-o575 (2007-10-06)
The crystal structure of a protected L-tyrosine, namely N-acetyl-L-tyrosine methyl ester monohydrate, C(12)H(15)NO(4).H(2)O, was determined at both 293 (2) and 123 (2) K. The structure exhibits a network of O-H...O and N-H...O hydrogen bonds, in which the water molecule plays
Sandra M Arriaga et al.
Clinical biochemistry, 42(9), 919-921 (2009-01-20)
To evaluate if unconjugated bilirubin (UB) inhibits C1 esterase activity. Esterase activity was evaluated by C1-mediated hydrolysis of N-acetyl-L-tyrosine ethyl ester, and binding of UB to C1r and C1s was assessed by dot-blot analysis. UB inhibited C1 enzymatic activity. C1r
Michaela Kreiner et al.
Biotechnology and bioengineering, 87(1), 24-33 (2004-06-24)
Recently, we reported a new high-activity biocatalyst for use in organic media termed protein-coated microcrystals (PCMC) (Kreiner et al. [2001] Chem Commun 12:1096-1097). These novel particles consist of water-soluble micron-sized crystalline particles coated with the given biocatalyst(s) and are prepared
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