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A2643

Sigma-Aldrich

α-Amylase from porcine pancreas

Type VII-A, DFP treated, ammonium sulfate suspension, ≥500 units/mg protein (E1%/280)

Sinonimo/i:

β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase,, porcine pancreas α-amylase

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About This Item

Classificazione EC (Enzyme Commission):
Numero MDL:
Codice UNSPSC:
12352204

Tipo

Type VII-A

Stato

ammonium sulfate suspension

Attività specifica

≥500 units/mg protein (E1%/280)

Temperatura di conservazione

2-8°C

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Descrizione generale

Molecular mass: 51-54 kDa.
α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.

Definizione di unità

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Stato fisico

Suspension in 3.2 M (NH4)2SO4, pH 6.1

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Pepsina powder, ≥250 units/mg solid

Sigma-Aldrich

P7000

Pepsina

Fushan Liu et al.
Journal of experimental botany, 60(15), 4423-4440 (2009-10-07)
The amylose extender (ae(-)) mutant of maize lacks starch branching enzyme IIb (SBEIIb) activity, resulting in amylopectin with reduced branch point frequency, and longer glucan chains. Recent studies indicate isozymes of soluble starch synthases form high molecular weight complexes with
Buford L Nichols et al.
The Journal of nutrition, 139(4), 684-690 (2009-02-06)
Starch is the major source of food glucose and its digestion requires small intestinal alpha-glucosidic activities provided by the 2 soluble amylases and 4 enzymes bound to the mucosal surface of enterocytes. Two of these mucosal activities are associated with
Wiesław Jerzy Cubała et al.
Journal of affective disorders, 157, 14-17 (2014-03-04)
Altered monoamine neurotransmission accompanied by hypothalamic-pituitary-adrenal axis dysfunction and autonomic nervous system hyperactivity have been associated with major depressive disorder (MDD). Salivary α-amylase (sAA) is indicative of autonomic activation and reflects central noradrenergic activity. Scarce studies on sAA in MDD
Heidemarie K Laurent et al.
Psychosomatic medicine, 75(8), 744-750 (2013-10-01)
Neurotrophins such as nerve growth factor (NGF) may represent a stress-responsive system complementing the better known neuroendocrine (hypothalamic-pituitary-adrenal axis) and autonomic nervous system, but there is little evidence for NGF response to acute stress in humans because noninvasive measures have
Sarika Jain et al.
The American journal of surgical pathology, 37(12), 1824-1830 (2013-11-15)
Chromophobe renal cell carcinoma (ChRCC) and oncocytoma present with a perplexing overlap of morphologic and immunohistochemical features. ChRCC have deletions in the 1p21.1 region including the amylase α-1A gene (AMY1A). No such deletions are found in oncocytoma. Instead, oncocytomas shared

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