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10065

Sigma-Aldrich

α-Amylase from Aspergillus oryzae

powder, ~30 U/mg

Sinonimo/i:

1,4-α-D-Glucan-glucanohydrolase

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About This Item

Classificazione EC (Enzyme Commission):
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Prezzi e disponibilità al momento non sono disponibili

Stato

powder

Livello qualitativo

Attività specifica

~30 U/mg

PM

Mr ~51000

Temperatura di conservazione

2-8°C

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Descrizione generale

Microbial amylases are exoenzymes and are used in several industrial applications, such as production of bread, maltose syrups, and fermentation of soya sauce, miso etc. α-Amylase isolated from Aspergillus oryzae has been found to have molecular weights of 51kDa (sedimentation and diffusion) and 49kDa (gel filtration).[1]

Applicazioni

α-Amylase has been used:
  • as a control enzyme in agar plate-based and carboxymethylcellulose-based clearing assays to screen cellulase activity[2]
  • for the hydrolysis of starch to explore the the role of wheat starch in frozen dough[3]
  • to inhibit Staphylococcus aureus biofilms[4]

Azioni biochim/fisiol

Aspergillus oryzae α-amylase (Ao α-amylase) enzyme catalyzes the hydrolysis of the α-1,4 glycosidic bonds in soluble starches and related subsrates. These substrates are broken down to release short oligosaccharides and α-limit dextrins.[5]

Definizione di unità

1 U corresponds to the amount of enzyme which liberates 1 μmol maltose per minute at pH 6.0 and 25°C (starch acc. to Zulkowsky, Cat. No. 85642, as substrate).

Altre note

For the determination of fats in food[6]; Application in (selective) hydrolysis/condensation of glycosidic bonds[7].

Pittogrammi

Health hazardExclamation mark

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Acute Tox. 4 Oral - Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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