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FLAGIPT1

Millipore

FLAG® Immunoprecipitation Kit

Sinonimo/i:

Anti-ddddk, Anti-dykddddk

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1 KIT
CHF 1’110.00

CHF 1’110.00


Spedizione prevista il26 marzo 2025



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Cambia visualizzazione
1 KIT
CHF 1’110.00

About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

CHF 1’110.00


Spedizione prevista il26 marzo 2025


impiego

 kit sufficient for 50 reactions

Livello qualitativo

Classi chimiche degli analiti

proteins (FLAG® Affinity Gels)

tecniche

immunoprecipitation (IP): suitable

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Descrizione generale

Sigma′s FLAG Immunoprecipitation kit allows a rapid and efficient immunoprecipitation and elution of an active FLAG-tagged protein. The immunoprecipitation is performed with the ANTI-FLAG M2 affinity gel, which is a highly specific monoclonal antibody covalently attached to agarose resin. The use of affinity resin allows an efficient binding of FLAG-tagged proteins without the need for preliminary steps and calibrations. The immunoprecipitated FLAG-tagged proteins can be efficiently eluted from the resin with acidic conditions or by competition with FLAG peptide. The immunoprecipitated proteins can be detected for their size, post-translational modifications, and interactions on gel electrophoresis, and by activity assays.

Applicazioni

Learn more product details in our FLAG® application portal.

Caratteristiche e vantaggi

  • Utilizes highly specific ANTI-FLAG M2 affinity gel
  • No preliminary steps or calibrations
  • 3X FLAG peptide provides efficient and gentle elution through direct competition

Altre note

All prodedures to be performed at 2-8°C.

Note legali

3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS

  • A2220ANTI-FLAG M2 affinity gel 1 mLSDS

  • SAE01943X FLAG peptide 1 mgSDS

Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Aquatic Chronic 3 - Eye Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids


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Samer Swedan et al.
Journal of virology, 85(19), 10090-10100 (2011-07-29)
Human respiratory syncytial virus (RSV), a major cause of severe respiratory diseases, efficiently suppresses cellular innate immunity, represented by type I interferon (IFN), using its two unique nonstructural proteins, NS1 and NS2. In a search for their mechanism, NS1 was
Mateusz Wydro et al.
Nucleic acids research, 38(11), 3732-3742 (2010-02-11)
Mammalian mitochondria contain their own genome that is almost fully transcribed from both strands, generating polycistronic RNA units that are processed and matured. The mitochondrial mRNA is modified by oligo- or polyadenylation at the 3' termini, but the exact function
Andrew S Terker et al.
American journal of physiology. Renal physiology, 315(4), F781-F790 (2018-02-08)
With no lysine kinase 4 (WNK4) is essential to activate the thiazide-sensitive NaCl cotransporter (NCC) along the distal convoluted tubule, an effect central to the phenotype of familial hyperkalemic hypertension. Although effects on potassium and sodium channels along the connecting
Mercedes Rodriguez-Teja et al.
Molecular cancer research : MCR, 13(3), 538-547 (2014-11-09)
Epithelial cell-cell contacts maintain normal glandular tissue homeostasis, and their breakage can trigger epithelial-to-mesenchymal transition (EMT), a fundamental step in the development of metastatic cancer. Despite the ability of C-type lectin domains (CTLD) to modulate cell-cell adhesion, it is not
Kalpana Kannan et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(11), E1272-E1277 (2015-03-04)
High-grade serous ovarian cancer (HGSC) is among the most lethal forms of cancer in women. Excessive genomic rearrangements, which are expected to create fusion oncogenes, are the hallmark of this cancer. Here we report a cancer-specific gene fusion between BCAM

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