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79760

Sigma-Aldrich

Ftaldialdeide

for fluorescence, ≥99.0% (HPLC)

Sinonimo/i:

Dicarbossaldeide o-ftalica, o-ftalaldeide, Benzene-1,2-dicarbossaldeide, OPA

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1 G
CHF 63.00
5 G
CHF 190.00
50 G
CHF 556.00

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1 G
CHF 63.00
5 G
CHF 190.00
50 G
CHF 556.00

About This Item

Formula empirica (notazione di Hill):
C8H6O2
Numero CAS:
643-79-8
Peso molecolare:
134.13
Beilstein:
878317
Numero CE:
211-402-2
Numero MDL:
MFCD00003335
Codice UNSPSC:
12352201
ID PubChem:
57652816
NACRES:
NA.32

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Grado

for fluorescence

Saggio

≥99.0% (HPLC)

Stato

solid

Impurezze

≤0.5% phthalic acid

Punto di fusione

54-56 °C

Fluorescenza

λex 334 nm; λem 455 nm (Thiol Adduct)
λex 340 nm; λem 450 nm in reaction buffer (with glycine)

Compatibilità

suitable for fluorescence

Temperatura di conservazione

2-8°C

Stringa SMILE

O=Cc1ccccc1C=O

InChI

1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H
ZWLUXSQADUDCSB-UHFFFAOYSA-N

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Descrizione generale

Phthaldialdehyde, also known as o-Phthalaldehyde or OPA, is a pre-column (HPLC) derivatization reagent. OPA reacts with primary amino acids (AA) except cystine to form highly fluorescent adducts. The fluorescence of the derivatives is monitored at excitation and emission wavelengths of 345nm and 455nm, respectively. The HPLC technique efficiently separates the OPA-AA derivatives.[1]

Applicazioni

Phthaldialdehyde or OPA determines amino acids in proteins from animal tissue and food samples. Quantification of amino acids adducts of tyramine and phenylethylamine produced by lactic acid bacteria used OPA as the pre-column derivative reagent for HPLC.[2] Intracellular forms of glutathione (GSH/GSSG) are investigated using o-Phthalaldehyde as the pre-column fluorescent agent.[3]

Azioni biochim/fisiol

La ftaldialdeide può essere utilizzata nelle separazioni HPLC per la derivatizzazione pre-colonna degli amminoacidi. Può anche essere impiegata per le misurazioni mediante citometria a flusso dei gruppi tiolici delle proteine.

Avvertenze

Danger

Indicazioni di pericolo

H301,H314,H317,H335,H410

Classi di pericolo

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

269.6 °F - closed cup

Punto d’infiammabilità (°C)

132 °C - closed cup

Dispositivi di protezione individuale

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
BCCM0599
BCCJ0409
BCCF5794
BCCC3481
BCCB5919

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José María Landete et al.
International journal of food microbiology, 115(3), 364-368 (2007-02-20)
The ability of wine lactic acid bacteria to produce tyramine and phenylethylamine was investigated by biochemical and genetic methods. An easy and accurate plate medium was developed to detect tyramine-producer strains, and a specific PCR assay that detects the presence
Hanne Vissenaekens et al.
Food research international (Ottawa, Ont.), 145, 110430-110430 (2021-06-12)
Flavonoid bioavailability and bioactivity is associated with interindividual variability, which is partially due to differences in health status. Previously, it was demonstrated that cellular stress, especially mitochondrial stress, increases intracellular quercetin uptake and this is associated with beneficial health effects.
Zhaolai Dai et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 964, 116-127 (2014-04-16)
Studies of protein nutrition and biochemistry require reliable methods for analysis of amino acid (AA) composition in polypeptides of animal tissues and foods. Proteins are hydrolyzed by 6M HCl (110°C for 24h), 4.2M NaOH (105°C for 20 h), or proteases.
Yun-Bin Han et al.
Applied microbiology and biotechnology, 99(16), 6715-6726 (2015-02-17)
Sphingolipid ceramide N-deacylase (SCDase) catalyzes reversible reactions in which the amide linkage in glycosphingolipids is hydrolyzed or synthesized. While SCDases show great value for the enzymatic synthesis of glycosphingolipids, they are relatively poorly characterized enzymes. In this work, the enzymatic
I Molnár-Perl
Journal of chromatography. A, 913(1-2), 283-302 (2001-05-18)
An overview is presented of HPLC methods currently in use to determine amino acids as their o-phthaldialdyde derivatives in the presence of various SH-group-containing additives. Crucial points that proved to influence the stability of the amino acid OPA derivatives have
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