09978

Solfato d′ammonio

BioUltra, ≥99.0% (T)

• Sinonimo/i: Mascagnite
• Formula condensata: (NH₄)₂SO₄
• Numero CAS: 7783-20-2
• Peso molecolare: 132.14
• Numero CE: 231-984-1
• Numero MDL: MFCD00003391
• Codice UNSPSC: 12352314
• eCl@ss: 38030408
• ID PubChem: 329748870
• NACRES: NA.26

Proprietà

• Nome Commerciale: BioUltra
• Saggio: ≥99.0% (T)
• Stato: crystals
• Impurezze: insoluble matter, passes filter test
• Residuo alla calcinazione: ≤0.01% (as SO₄)
• Perdita: ≤0.1% loss on drying, 110 °C
• pH: 5.0-6.0 (25 °C, 1 M in H₂O)
• Punto di fusione: >280 °C (dec.) (lit.)
• Solubilità: H₂O: 1 M at 20 °C, clear, colorless
• Densità: 1.77 g/mL at 25 °C (lit.)
• Anioni in tracce: chloride (Cl^-): ≤5 mg/kg; nitrate (NO₃^-): ≤50 mg/kg; phosphate (PO₄^3-): ≤5 mg/kg
• Cationi in tracce: Al: ≤5 mg/kg; As: ≤0.2 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤1 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤2 mg/kg; Fe: ≤2 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤50 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤2 mg/kg; Sr: ≤5 mg/kg; Zn: ≤1 mg/kg
• λ: 1 M in H₂O
• Assorbanza UV: λ: 260 nm A_max: ≤0.037; λ: 280 nm A_max: ≤0.030
• Stringa SMILE: N.N.OS(O)(=O)=O; N.N.OS(O)(=O)=O
• InChI: 1S/2H3N.H2O4S/c;;1-5(2,3)4/h2*1H3;(H2,1,2,3,4); BFNBIHQBYMNNAN-UHFFFAOYSA-N

Descrizione

Descrizione generale

Ammonium sulfate is naturally present as a rare mineral mascagnite in volcanic fumaroles and due to coal fires in some dumps.

Applicazioni

Ammonium sulfate has been used:

• to prepare mobile phase A for hydrophobic interaction chromatography (HIC)
• as reference material for the thermal decomposition of NH₄NO₃ (S1–S6) thermal decomposition to N₂O (S1-N₂O–S6-N₂O)
• to prepare the stock solution for M63 medium
• as a reagent in molecular biology and chromatography
• in the precipitation and fractionation of proteins
• in the purification of antibodies
• for the crystallographic analysis of nucleic acids and proteins
• in high-performance liquid chromatography (HPLC) analysis of proteins, such as in hydrophobic interaction chromatography
• for Sepharose chromatography of tRNAs using reverse salt gradients
• in long PCR buffer, in PCR lysis solution, and second-strand cDNA synthesis
• HPLC analysis of phospholipids

Azioni biochim/fisiol

Ammonium sulfate is an inorganic salt, which is mostly used as a soil fertilizer. It is also used as a flame retardant since it elevates the combustion temperature of the material and lowers maximum weight loss rates. This leads to an upsurge in the production of residue or char.

Altre note

The most commonly used salt for the fractional precipitation of proteins due to its solubility and effectiveness[1]; Protein crystallization[2]; Sepharose^™ chromatography of tRNAs using reverse salt gradients[3].

Note legali

Sepharose is a trademark of Cytiva

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 13 - Non Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)