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Documenti fondamentali

270733

Sigma-Aldrich

Acqua

Water
1 of 1 reviewers received a sample product or took part in a promotion

suitable for HPLC

Sinonimo/i:

Acqua, Ossido di diidrogeno

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About This Item

Formula condensata:
H2O
Numero CAS:
Peso molecolare:
18.02
Beilstein:
2050024
Numero CE:
Numero MDL:
Codice UNSPSC:
12190000
ID PubChem:
NACRES:
NA.21
Prezzi e disponibilità al momento non sono disponibili

Grado

HPLC grade

Livello qualitativo

Densità del vapore

<1 (vs air)

Tensione di vapore

3 mmHg

Stato

liquid

tecniche

HPLC: suitable

Residuo dopo evaporazione

<0.0003%

Indice di rifrazione

n20/D 1.34 (lit.)

pH

6.0-8.0 (25 °C)

P. ebollizione

100 °C (lit.)

Punto di fusione

0 °C (lit.)

Densità

1.000 g/mL at 3.98 °C (lit.)

λ

H2O reference

Assorbanza UV

λ: 205 nm Amax: 0.01
λ: 210 nm Amax: 0.01
λ: 250-400 nm Amax: 0.005

Stringa SMILE

O

InChI

1S/H2O/h1H2
XLYOFNOQVPJJNP-UHFFFAOYSA-N

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Descrizione generale

L′acqua per HPLC è in genere usata come solvente per l′eluizione isocratica sia in cromatografia anionica sia in cromatografia cationica. Inoltre, viene usata come componente per la preparazione di soluzioni madre standard e per l′evaporazione in corrente di azoto in bagno ad acqua per la concntrazione del campione prima dell′analisi LC-MS-MS.[1] [2]

Applicazioni

L′acqua viene usata come:
  • Solvente nella preparazione di soluzioni tampone durante il processo di lisi cellulare[3]
  • Componente della fase mobile nel corso della rivelazione mediante HPLC con rivelatore UV a serie di diodi per la separazione e la rivelazione degli analiti.[4]

Confezionamento

M-Bottle for Solvents
As a global leader in lab reagents, we are constantly looking for new ways to optimize the safety of our products. The newly developed 4L solvent bottle design features advanced sealing technology that eliminates leaks to make the handling of solvents safer and more convenient than ever before.
See all the new features here!

Nota sulla preparazione

Prodotto filtrato da un filtro da 0,2 μm

Altre note

Nota importante
L′articolo codice 270733-6X1L uscirà di produzione. Si prega di ordinare la bottiglia singola 270733-1L che è fisicamente identica e presenta esattamente le stesse specifiche.

Prodotti consigliati

Scopri i reagenti LiChropur ideali per analisi HPLC o LC-MS

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Certificati d'analisi (COA)

Lot/Batch Number

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Protocolli

A guide to create solvent systems used for the thin-layer chromatography assay of Novabiochem products.

Questions

1–8 of 8 Questions  
  1. Is this water suitable for lal test method ? Is pyrogen free ? Is there a specification?

    1 answer
    1. This product is not suitable for the Limulus Amebocyte Lysate test method (LAL). Specifications for this product are available in the documents section of the webpage, but pyrogen levels are not specified. This product is not certified as pyrogen-free and is not sterile. This product is intended for use as a solvent for High-Performance Liquid Chromatography mobile phases.

      Helpful?

  2. Will dispensing small quantities and resealing the container lead to air dissolving in the water? I intend to use 50ml-100ml for my experiments.

    1 answer
    1. Opening a container that is not isolated from the atmosphere could results in ambient air dissolving in the water remaining inside the container. The rate of of air introduction would need to be monitored for a given set of conditions such as temperature, air exchange in the laboratory, and relative humidity.

      Helpful?

  3. Do you know the resistivity value @ 25°C?

    1 answer
    1. The resistivity of this product has not been determined.

      Helpful?

  4. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  5. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  6. How is this water purified? is it distilled?

    1 answer
    1. The water purification process involves several steps:
      • The water initially passes through a carbon exchange tank to remove organic compounds.
      • It is then filtered using a 1μm PE cartridge filter to remove any remaining impurities.
      • Inorganic compounds are eliminated through deionization tanks, ensuring high purity. The resistivity of the water is checked during this stage.
      •The water undergoes sterilization using UV light to eliminate any microorganisms.
      • Finally, the water is filtered through a 0.2μm cartridge filter, and the resulting HPLC grade water is packaged in glass bottles.
      Note: This information provides an overview of the typical water purification process and may vary depending on specific applications or systems.

      Helpful?

  7. Do you know the concentration of TOC in this water?

    1 answer
    1. The concentration of TOC in this product is not determined. Product 78533, Water for TOC Analysis, may be of interest.
      Please see the link below to review this product option:
      https://www.sigmaaldrich.com/product/sial/78533

      Helpful?

  8. Is this product (HPLC grade water #270733) DNase-, RNase- and protease-free?

    1 answer
    1. The item is not tested for the presence of DNA, RNA, and protease.

      Please see the links below to review product options that include this testing:
      W4502
      https://www.sigmaaldrich.com/US/en/product/sigma/w4502
      95284
      https://www.sigmaaldrich.com/US/en/product/sigma/95284

      Note that the above options are NOT tested for suitability in HPLC.

      Helpful?

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

    • Reviews 3
    • Votes 9
    5 out of 5 stars.

    Good, pure water

    Water was high purity, HPLC grade. We didn't actually use it for HPLC but it seemed very clean and pure for our purposes.

    Helpful?

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