11699105001

PCR Buffer Without MgCl₂, 10x concentrated

solution, pkg of 3 × 1 mL

• Sinonimo/i: buffer
• Codice UNSPSC: 41105600

Proprietà

• Stato: solution
• Livello qualitativo: 100
• Confezionamento: pkg of 3 × 1 mL
• Produttore/marchio commerciale: Roche
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

The PCR buffer without MgCl₂, in combination with the MgCl₂ stock solution, is used for the individual adjustment of the Mg²⁺ concentration in the PCR reaction. In most applications a concentration of 1.5mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg²⁺ concentration to increase the specificity and yield of the PCR reaction.The buffer is ten-fold concentrated and should be used in combination with the MgCl₂ stock solution.

Applicazioni

The PCR buffer without MgCl₂ has been used for the amplification of specific DNA fragments by the polymerase chain reaction (PCR).[1][2]

Stato fisico

Solution, 100 mM Tris-HCl, 500 mM KCl (pH 8.3 at 20 °C)

Altre note

For general laboratory use.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash