11633716001
Roche
Anti-Digoxigenin-POD (poly), Fab fragments
from sheep
Sinonimo/i:
anti-digoxigenin, digoxigenin
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About This Item
Codice UNSPSC:
12352203
Clone:
polyclonal
application:
citations:
34
Prodotti consigliati
Origine biologica
sheep
Livello qualitativo
Coniugato
peroxidase conjugate
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Stato
lyophilized
Confezionamento
pkg of 50 U
Produttore/marchio commerciale
Roche
Isotipo
IgG
Temperatura di conservazione
2-8°C
Descrizione generale
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life. This product contains Fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to polymerized horseradish peroxidase.
Specificità
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Applicazioni
Anti-Digoxigenin-POD (poly), Fab fragments has been used for the detection of digoxigenin-labeled compounds using:
Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.
- whole-mount in situ hybridization
- fluorescence in situ hybridization
- enzyme linked immunosorbent assay (ELISA)
- dot blot
- Southern blot
- western blot
- double labeling experiments
- lectin blots
Anti-Digoxigenin-POD (poly), Fab fragments give considerably higher signal-to-noise values compared to anti-Digoxigenin-Fab fragment conjugated to the unpolymerized horseradish peroxidase in ELISA applications. It is specifically useful when high sensitivity is required.
Anti-Digoxigenin-POD (poly), Fab fragments have not been evaluated in immunohistochemistry.
Nota sulla preparazione
Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
- Dot blot: 50 to 100 mU/ml
- ELISA: 2 to 50 mU/ml
- Western blot: 50 to 100 mU/ml
- Southern blot: 50 to 100 mU/ml
Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Ricostituzione
Add 1 ml double-distilled water to a final concentration of 50 U/ml.
Altre note
For life science research only. Not for use in diagnostic procedures.
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Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Skin Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
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Articoli
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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