11207741910
Roche
Anti-Digoxigenin-Fluorescein, Fab fragments
from sheep
Sinonimo/i:
anti-digoxigenin, digoxigenin
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About This Item
Codice UNSPSC:
12352203
Prodotti consigliati
Origine biologica
sheep
Livello qualitativo
Coniugato
fluorescein conjugate
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Forma fisica
lyophilized
Confezionamento
pkg of 200 μg
Produttore/marchio commerciale
Roche
Isotipo
IgG
Temperatura di conservazione
2-8°C
Descrizione generale
Anti-Digoxigenin-Fluorescein, Fab fragments has fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to fluorescein.
Specificità
The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.
Applicazioni
Anti-Digoxigenin-Fluorescein, Fab fragments are used for the detection of digoxigenin-labeled compounds using:
- Digoxigenin-labeled sugars in glycoconjugate research
- Fluorescent in situ hybridization (FISH)
- Immunohistocytochemistry
- In situ hybridization
Nota sulla preparazione
- Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:Detection of digoxigenin-labeled sugars in glycoproteins: 50 to 20μg/ml
- Fluorescent in situ hybridization (FISH): 1 to 20μg/ml
- Immunohistocytochemistry: 20 to 50μg/ml
- In situ hybridization: 20 to 50μg/ml
The anti-DIG anitbody is highly specific and sensitive. Therefore, for FISH, a concentration of 1μg/ml is sufficient, and only in case of critical probes, this concentration may be raised to <10μg/ml (with a distinct background to be expected).
Working solution: Reconstitution
Add 1 ml double-distilled water to a final concentration of 200μg/ml.
Blocking:
PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore, pH can be increased up to pH 8.5 to 9.0.
Storage conditions (working solution): Always prepare fresh!
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.
Ricostituzione
Add 1 ml double-distilled water to a final concentration of 200 μg/ml.
Altre note
For life science research only. Not for use in diagnostic procedures.
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Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3 - Skin Sens. 1
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
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Articoli
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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