MABT341

Anti-CD226/DNAM-1 Antibody, clone NewE1 (Azide Free)

clone NewE1, from mouse

• Sinonimo/i: CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD_antigen=CD226
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: NewE1, monoclonal
• Reattività contro le specie: human
• tecniche: activity assay: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_006557
• N° accesso UniProt: Q15762
• Condizioni di spedizione: dry ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CD226(10666)

Descrizione

Descrizione generale

CD226/DNAM1 is a T cell expressed plasma membrane receptor protein that plays multiple roles in lymphocyte signaling, intercellular adhesion, lymphokine secretion and cytotoxic T-cell and NK cell mediated killing. CD226 activates natural killer (NK) cell-mediated cytotoxicity and is a key player in the immune synapse between killer T cells and targets. Also, because it recognizes CD155 and nectins CD226/DNAM1 is being examined as a potential anti-cancer agent because soluble CD226 directly inhibits tumor growth by binding CD155 expressed on tumor cells and down regulating proliferation. CD226/DNAM-1 is expressed on Th1, but not Th2 or Th0 cells, it is also expressed on NK cells, CD8+ T-cells, but not developing T cells. CD226/DNAM-1 is also expressed on monocytes and platelets.

Immunogeno

Recombinant protein corresponding to human CD226/DNAM-1.

Applicazioni

Activity Assay Analysis: A representative lot detected CD226/DNAM-1 which induces platelet activation and aggregation (Sherrington, P.D., et al. (1997). J. Biol. Chem. 272:21735-21744).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CD226/DNAM-1 in isolated surface proteins from Jurkat and platelets (Sherrington, P.D., et al. (1997). J. Biol. Chem. 272:21735-21744).
MABT341 works by western blotting under both non-reducing and reducing conditions.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-CD226/DNAM-1 Antibody, clone NewE1 (Azide Free) is validated for use in Western Blotting and Immunoprecipitation and Activity Assay for the detection of CD226/DNAM-1.

Qualità

Evaluated by Western Blotting in CD226 Transfected CHO cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CD226/DNAM-1 in 10 µg of CD226 Transfected CHO cell lysate.

Descrizione del bersaglio

~39 kDa observed

Stato fisico

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Stoccaggio e stabilità

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable