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MABT29

Sigma-Aldrich

Anti-Ninein Antibody, clone 79-160-7

clone 79-160-7, from mouse

Sinonimo/i:

ninein (GSK3B interacting protein), Glycogen synthase kinase 3 beta-interacting protein, ninein centrosomal protein, GSK3B-interacting protein

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100 μG
CHF 449.00

CHF 449.00


Spedizione prevista il22 maggio 2025


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Cambia visualizzazione
100 μG
CHF 449.00

About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

CHF 449.00


Spedizione prevista il22 maggio 2025


Richiedi un ordine bulk

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified antibody

Tipo di anticorpo

primary antibodies

Clone

79-160-7, monoclonal

Reattività contro le specie

human

tecniche

immunoprecipitation (IP): suitable
western blot: suitable

Isotipo

IgG2aκ

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... NIN(51199)

Descrizione generale

Ninein is a coiled-coil, centrosomal protein that is a necessary component in microtubule minus-end anchorage and positioning, and may have a role as a maturation factor for centrosomes. It may also be involved in microtubule nucleation. More recent studies have identified ninein as a crucial component in the formation of blood vessels. Expression of ninein is ubiquitous, but significantly higher levels have been observed in skeletal muscle and heart tissue. There have been 8 isoforms characterized due to alternative splicing.

Immunogeno

Recombinant protein corresponding to human Ninein.

Applicazioni

Anti-Ninein Antibody, clone 79-160-7 is an antibody against Ninein for use in WB & IP.
Immunocytochemistry Analysis: 1:500 dilution of the antibody has been shown to detect Ninein in HeLa and A431 cells.

Immunoprecipitation Analysis: The antibody has been shown to immunoprecipitate Ninein in L929 cells. (Delgehyr, 2005).

Qualità

Evaluated by Western Blot in Hek293 cell lysate.

Western Blot Analysis: 1 µg/mL of the antibody detected Ninein in 10 µg of Hek293 cell lysate.

Descrizione del bersaglio

~ 250 kDa observed MW. An unidentified nonspecific band appears at ~ 50 kDa

Stato fisico

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Izumi Dateyama et al.
Journal of cell science, 132(2) (2018-12-24)
The primary cilium, a solitary protrusion from most mammalian cells, functions as a cell sensor by receiving extracellular signals through receptors and channels accumulated in the organelle. Certain G-protein-coupled receptors (GPCRs) specifically localize to the membrane compartment of primary cilia.
Dong Hyun Kim et al.
Cell death & disease, 10(8), 570-570 (2019-07-31)
The initiation of centrosome duplication is regulated by the Plk4/STIL/hsSAS-6 axis; however, the involvement of other centrosomal proteins in this process remains unclear. In this study, we demonstrate that Cep131 physically interacts with Plk4 following phosphorylation of residues S21 and
Xavier Gaume et al.
Cell cycle (Georgetown, Tex.), 14(6), 902-919 (2015-01-16)
Nucleolin is a pleiotropic protein involved in a variety of cellular processes. Although multipolar spindle formation has been observed after nucleolin depletion, the roles of nucleolin in centrosome regulation and functions have not been addressed. Here we report using immunofluorescence
Tatsuo Miyamoto et al.
The EMBO journal, 39(12), e103499-e103499 (2020-05-06)
Primary cilia are antenna-like organelles on the surface of most mammalian cells that receive sonic hedgehog (Shh) signaling in embryogenesis and carcinogenesis. Cellular cholesterol functions as a direct activator of a seven-transmembrane oncoprotein called Smoothened (Smo) and thereby induces Smo
Aamir Ali et al.
Nature communications, 14(1), 289-289 (2023-01-27)
Organization of microtubule arrays requires spatio-temporal regulation of the microtubule nucleator γ-tubulin ring complex (γTuRC) at microtubule organizing centers (MTOCs). MTOC-localized adapter proteins are thought to recruit and activate γTuRC, but the molecular underpinnings remain obscure. Here we show that

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