MAB339
Anti-GABA A Receptor α1 chain Antibody, NT, clone BD24
clone BD24, Chemicon®, from mouse
Sinonimo/i:
Anti-DEE19, Anti-ECA4, Anti-EIEE19, Anti-EJM, Anti-EJM5
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
BD24, monoclonal
Reattività contro le specie
human, pig, avian, bovine
Non deve reagire con
feline, rat
Produttore/marchio commerciale
Chemicon®
tecniche
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG1
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... GABRA1(2554)
Specificità
Reacts with the alpha 1-chain of the GABAA receptor in bovine and human but not rat
Immunogeno
Epitope: N-terminus
Purified GABA/benzodiazepine receptor from bovine cortex.
Applicazioni
Anti-GABA A Receptor α1 chain Antibody, N-terminus, clone BD24 is an antibody against GABA A Receptor α1 chain for use in IH, IP & WB.
Immunohistochemistry: 10-20 μg/mL * See protocol below.
Western blot: 20 μg/mL
Immunoprecipitation
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB339
IMMUNOHISTOCHEMISTRY
1) Fresh tissue (human) should be used (3-6 hours postmortem). The tissue should be immersion fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50 mm sections and store in cryoprotective solution at -15°C.
2) Sections should be incubated floating in suitable small vials.
3) Block endogenous peroxidase with 100 mmol/L Tris-HCl, 150 mmol/L NaCl, pH 7.4 (Buffer A) containing 3% H2O2 (v/v) and 10% methanol for 20 min at room temperature.
4) Wash sections in Buffer A. Incubate sections in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v).
5) Wash sections in Buffer A. Incubate sections with MAB339 (diluted 10-20 mg/mL in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v) for 12-36 hours at +4°C
6) Wash sections in Buffer A.
7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
8) Wash sections in Buffer A.
9) Mount sections on chrome alum-coated slides, dry, dehydrate, and apply coverslips.
Western blot: 20 μg/mL
Immunoprecipitation
Optimal working dilutions must be determined by end user.
APPLICATION NOTES FOR MAB339
IMMUNOHISTOCHEMISTRY
1) Fresh tissue (human) should be used (3-6 hours postmortem). The tissue should be immersion fixed in 2% paraformaldehyde/0.1% glutaraldehyde. Prepare 50 mm sections and store in cryoprotective solution at -15°C.
2) Sections should be incubated floating in suitable small vials.
3) Block endogenous peroxidase with 100 mmol/L Tris-HCl, 150 mmol/L NaCl, pH 7.4 (Buffer A) containing 3% H2O2 (v/v) and 10% methanol for 20 min at room temperature.
4) Wash sections in Buffer A. Incubate sections in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v).
5) Wash sections in Buffer A. Incubate sections with MAB339 (diluted 10-20 mg/mL in Buffer A containing 5% fetal bovine serum (v/v) and 0.1-0.5% Triton X-100 (v/v) for 12-36 hours at +4°C
6) Wash sections in Buffer A.
7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).
8) Wash sections in Buffer A.
9) Mount sections on chrome alum-coated slides, dry, dehydrate, and apply coverslips.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Stato fisico
Format: Purified
Liquid. Buffer = 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
Stoccaggio e stabilità
Maintain lyophilized material at +2-8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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