MAB3300
Anti-TIMP-1 Antibody, clone 7-6C1
clone 7-6C1, Chemicon®, from mouse
Sinonimo/i:
Tissue Inhibitor of Metalloproteinase-1
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
7-6C1, monoclonal
Reattività contro le specie
rat, human, bovine
Non deve reagire con
mouse
Produttore/marchio commerciale
Chemicon®
tecniche
ELISA: suitable
immunohistochemistry: suitable
western blot: suitable
Isotipo
IgG1κ
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... TIMP1(7076)
Descrizione generale
Tissue Inhibitors of Metalloproteinases (TIMPs) inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-1 and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kDa type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-1 levels in quiescent cells and tissues is low (pg/ml), and stimulation with the phorbol ester TPA or protein concentration is often needed to visualize protein bands. In addition, cell types differ greatly in the amount of TIMP-1 produced.
Specificità
MAB3300 is a purified mouse monoclonal antibody to bovine tissue inhibitor of metalloproteinases-1 (bovine TIMP-1). The antibody specifically reacts with bovine TIMP-1 and cross-reacts with human and rat TIMP-1, but not with mouse TIMP-1. By western blot the antibody recognizes a glycoprotein of 28.5 kDa, which is identified as tissue inhibitor of metalloproteinases-1 (TIMP-1).
Immunogeno
Bovine TIMP-1
Applicazioni
Detect TIMP-1 using this Anti-TIMP-1 Antibody, clone 7-6C1 validated for use in ELISA, WB, IH.
Immunoblotting: 1:1000, identifies a band of approximately 30KDa. Note TIMP-1 is found in very low concentrations in most cells and tissues.Positive control is HT1080 cells treated with PMA or recombinant TIMP-1 protein CC3328.
Immunohistochemistry on frozen sections or acetone fixed tissues. Formalin fixed paraffin-embedded tissue sections are usually not reactive. PLP fixation is suggested as an alternative.
EIA
Optimal working dilutions must be determined by the end user.
Immunohistochemistry on frozen sections or acetone fixed tissues. Formalin fixed paraffin-embedded tissue sections are usually not reactive. PLP fixation is suggested as an alternative.
EIA
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
Linkage
Replaces: 04-1133
Stato fisico
Format: Purified
Purified immunoglobulin presented as a liquid in 0.1 M Na-Phosphate buffer, pH 7.0 containing protease free bovine serum albumin.
Stoccaggio e stabilità
Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles.
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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