Passa al contenuto
Merck
Tutte le immagini(1)

Documenti fondamentali

71296

Millipore

PhosphoSafe Extraction Reagent

Novagen′s PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state.

Sinonimo/i:

Protein Extraction Reagent

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali

Scegli un formato

25 ML
CHF 139.00
125 ML
CHF 511.00

CHF 139.00

Spedizione prevista il28 maggio 2025

Tutte le immagini(1)

Scegli un formato

Cambia visualizzazione
25 ML
CHF 139.00
125 ML
CHF 511.00

About This Item

Codice UNSPSC:
41106510
NACRES:
NA.77

CHF 139.00

Spedizione prevista il28 maggio 2025

Livello qualitativo

100

Stato

liquid

Produttore/marchio commerciale

Novagen®

Condizioni di stoccaggio

OK to freeze

tecniche

immunoblotting: suitable

applicazioni

sample preparation

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Categorie correlate

Descrizione generale





PhosphoSafe Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formula as CytoBuster Protein Extraction Reagent (Trim 2001), but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, β-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications. This reagent is provided in convenient 25-ml bottles (1 bottle for 71296-3, 5 bottles for 71296-4).

Caratteristiche e vantaggi

  • Phosphorylation state preserved during extraction
  • Compatible with kinase assays

Attenzione

Toxicity: Toxic (F)

Altre note

Trim, J.E. and Sawyer, D.L. 2001. inNovations12, 5.

Note legali

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 6

1 of 6

Ponceau S BioReagent, suitable for electrophoresis

Sigma-Aldrich

P3504

Ponceau S

Cocktail di inibitori delle proteasi for use with mammalian cell and tissue extracts, DMSO solution

Sigma-Aldrich

P8340

Cocktail di inibitori delle proteasi

Sample Buffer, Laemmli 2× Concentrate

Sigma-Aldrich

S3401

Sample Buffer, Laemmli 2× Concentrate

Tampone RIPA

Sigma-Aldrich

R0278

Tampone RIPA

Cocktail di inibitori delle proteasi cOmplete™ Tablets provided in EASYpacks

Roche

04693116001

Cocktail di inibitori delle proteasi cOmplete™

PhosSTOP™ sufficient for 10 10mL buffer preparations, sufficient for 20 10mL buffer preparations, suitable for tissue processing, suitable for immunoprecipitation (IP)

Roche

PHOSS-RO

PhosSTOP™

Adrienne R Gibson et al.
Cancer research, 80(7), 1401-1413 (2020-02-12)
Pharmacologic ascorbate treatment (P-AscH-, high-dose, intravenous vitamin C) results in a transient short-term increase in the flux of hydrogen peroxide that is preferentially cytotoxic to cancer cells versus normal cells. This study examines whether an increase in hydrogen peroxide is

Questions

1–2 of 2 Questions  

  1. what is recomended volume for 50mg tissue extraction? would you recomend adding protease ihnibitor too? could you share protocol for protein extraction from liver using this phosphosafe extraction reagent?

    1 answer

    1. A protocol for liver tissue particularly is not offered. Here is a protocol for tissue in general:

      Tissue homogenates are prepared by homogenizing tissue in ice-cold PhosphoSafe extraction buffer using 70 ul buffer/mg tissue. Homogenates are incubated at room temperature for 5 mins, sonicated on ice, and then subjected to centrifugation (spun at 16,000 X g, 5 mins, at 4 Celsius). The resulting supernatant is collected (the end-user will want to keep this, and can discard the insoluble pellet). Finally, protein concentration is determined by a protein assay (e.g., Bradford assay).

      To minimize proteolysis, extraction can be performed on ice, and protease inhibitors can be added.
      Please see the link below to access the full product user guide:
      https://www.emdmillipore.com/product/PhosphoSafe-Extraction-Reagent,EMD_BIO-71296#anchor_USP

      Helpful?

  2. I am doing protein isolation from the organic phase remaining after RNA isolation using Trizol/Chloroform. How can I use PhosphoSafe™ Extraction Reagent in this case?

    1 answer

    1. Phosphosafe has been tested for use in aqueous formats such as cell culture supernatants or lysates. The performance of the product in an organic phase following fractionation with Trizol has not been determined. To discuss further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Product Technical Inquires" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.

      Helpful?

Reviews

No rating value

Active Filters

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.