P3504

Ponceau S

Powder

• Sinonimo/i: 3-Hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt
• Formula empirica (notazione di Hill): C₂₂H₁₂N₄Na₄O₁₃S₄
• Numero CAS: 6226-79-5
• Peso molecolare: 760.57
• Colour Index: 27195
• Numero CE: 228-319-2
• Numero MDL: MFCD00003892
• Codice UNSPSC: 12171500
• ID PubChem: 24898418
• NACRES: NA.47

Proprietà

• Nome del prodotto: Ponceau S, BioReagent, suitable for electrophoresis
• Nome Commerciale: BioReagent
• Livello qualitativo: 200
• Stato: powder
• tecniche: electrophoresis: suitable
• Colore: red to very dark red
• Solubilità: H₂O: 1 mg/mL
• ε (coefficiente d’estinzione): ≥330000 at 518-522 nm in deionized water at 0.015 g/L
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: room temp
• Stringa SMILE: [Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;; KQHKSGRIBYJYFX-WNDMICSESA-J

Descrizione

Descrizione generale

Ponceau S is the sodium salt of a diazo dye. It is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. The stain is reversed with water washes and aids in subsequent immunological detection.[1]
The following are two common stain formulations:
0.1% Ponceau S (w/v) in 5% acetic acid
2% Ponceau S (w/v) in 30% TCA, 30% sulfosalicylic acid

Applicazioni

Ponceau S has been used for membrane staining (Western blotting.[2][3][4]

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves