616371

Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free

recombinant, expressed in Nicotiana, heat and acid-stable competitive, reversible inhibitor of serine proteases

• Sinonimo/i: Aprotinin, Bovine, Recombinant, Nicotiana sp., Animal-Free, Pancreatic Trypsin Inhibitor, Trypsin-Kallikrein Inhibitor, Kallikrein-Trypsin Inactivator, Antikrein, Basic Pancreatic Trypsin Inhibitor
• Formula empirica (notazione di Hill): C₂₈₄H₄₃₂N₈₄O₇₉S₇
• Numero CAS: 9087-70-1
• Peso molecolare: 6511.44
• Numero MDL: MFCD00130541
• Codice UNSPSC: 12352202
• Numero indice EU: 2329949
• NACRES: NA.77

Proprietà

• Ricombinante: expressed in Nicotiana
• Livello qualitativo: 200
• Descrizione: Merck USA index - 14, 757
• Saggio: ≥98% (SDS-PAGE)
• Stato: powder
• Attività specifica: ≥5.0 units/mg protein
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze
• Colore: white to off-white
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• Stringa SMILE: S1SCC2NC(=O)CNC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C5NC(=O)C(NC(=O)CNC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C(NC(=O)C9N(CCC9)C(=O)CNC(=O)C(NC(=O)C(NC(=O)C%11N(CCC%11)C(=O
• InChI: ZPNFWUPYTFPOJU-UHFFFAOYSA-N

Descrizione

Descrizione generale

Recombinant, bovine aprotinin supplied without animal-derived components. Aprotinin is a competitive, reversible inhibitor of proteolytic and esterolytic activity. A relatively heat- and acid-stable serine protease inhibitor. Forms a tight complex, blocking the active site of the enzyme. Effective at concentrations equimolar with protease. Inhibits several proteases, including coagulation factors in the prephase of blood clotting, tissue and leukocytic proteinases, chymotrypsin, trypsin (K_d = 5 x 10^-14 M), plasmin (K_d = 2.3 x 10^-10 M), and kallikrein (K_d = 1 x 10^-7 M). Proteases not inhibited by aprotinin include Factor Xa, thrombin, pepsin, papain, and carboxypeptidases A and B. Useful for protein purification and for extending the life of cells in culture by preventing proteolytic damage.

Azioni biochim/fisiol

Cell permeable: no

Kd = 5 x 10-14 M, 2.3 x 10-10 M, 1 x 10-7 M against trypsin, plasmin, and kallikrein, respectively

Primary Target
chymotrypsin

Product does not compete with ATP.

Reversible: yes

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One TIU (Trypsin Inhibitory Unit) will decrease the activity of two trypsin units by 50% where one trypsin unit will hydrolyze 1.0 µmole of Nα-benzoyl-L-Arginine-p-Nitroanilide (L-BAPNA) per minute at pH 7.8 at 25°C.

Stato fisico

Contains no animal-derived components.

Altre note

Deutscher, M.P., 1990. Methods Enzymol.182, 83.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable