324831
EGF human
≥95% (SDS-PAGE), recombinant, expressed in E. coli, solid, suitable for cell culture
Sinonimo/i:
EGF, Human, Recombinant, E. coli, Epidermal Growth Factor, Human, Recombinant, E. coli
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200 μG
CHF 608.00
CHF 608.00
Spedizione prevista il29 maggio 2025
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Cambia visualizzazione
200 μG
CHF 608.00
About This Item
CHF 608.00
Spedizione prevista il29 maggio 2025
Prodotti consigliati
Nome del prodotto
EGF, Human, Recombinant, E. coli,
Ricombinante
expressed in E. coli
Livello qualitativo
Descrizione
Merck USA index - 14, 3527
Saggio
≥95% (SDS-PAGE)
Stato
solid
Produttore/marchio commerciale
Calbiochem®
Condizioni di stoccaggio
OK to freeze
Impurezze
<1.0 EU/μg Endotoxin (EU/μg EGF)
Condizioni di spedizione
ambient
Temperatura di conservazione
−20°C
Descrizione generale
Recombinant, human epidermal growth factor (54 amino acids) expressed in E. coli. Identical to native human EGF except for an additional N-terminal methionine. Human EGF is 70% homologous to mouse EGF and has been shown to have similar biological activity. Mitogenic for a wide range of ectoderm- and endoderm-derived cells from tissue such as breast, cornea, epidermis, dermis, liver, pancreas, nerve, amnion, and adrenal medulla. Binding of EGF to its 170 kDa transmembrane receptor results in dimerization of the receptor, activation of the intrinsic receptor tyrosine kinase, and initiation of the complex signal transduction cascade that leads to cell proliferation and/or differentiation. Acts as a survival factor in preventing apoptosis.
Azioni biochim/fisiol
ED₅₀ = 100-400 pg/ml as measured by its ability to stimulate the ³H-thymidine incorporation in an EGF-responsive mouse fibroblast cell line, Balb/3T3.
Attenzione
Toxicity: Standard Handling (A)
Ricostituzione
Following reconstitution, aliquot and freeze (-20°C or -70°C) for long term storage or refrigerate (4°C) for short-term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C.
Reconstitute to a concentration of ≥10 µg/ml in 10 mM acetic acid containing ≥0.1% HSA or BSA.
Altre note
Merlo, G.R., et al. 1995. J. Cell Biol. 128, 1185.
Yoshida, T., et al. 1993. Brain Res. Dev.76, 147.
Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA88, 415.
Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
Downward, J., et al. 1984. Nature311, 483.
Gray, A., et al. 1983. Nature303, 722.
Scott, J., et al. 1983. Science221, 236.
Cohen, S. 1960. Proc. Natl. Acad. Sci. USA46, 302.
Yoshida, T., et al. 1993. Brain Res. Dev.76, 147.
Rubin, J.S., et al. 1991. Proc. Natl. Acad. Sci. USA88, 415.
Carpenter, G., and Wahl, M.I. 1990. In The Epidermal Growth Factor Family in Peptide Growth Factors and Their Receptors (Sporn, M.B., and Roberts, A.B., eds.) Springer-Verlag, New York, p 69.
Downward, J., et al. 1984. Nature311, 483.
Gray, A., et al. 1983. Nature303, 722.
Scott, J., et al. 1983. Science221, 236.
Cohen, S. 1960. Proc. Natl. Acad. Sci. USA46, 302.
Note legali
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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