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Sigma-Aldrich

DAPI, Dihydrochloride

Cell-permeable DNA-binding dye.

Sinonimo/i:

DAPI, Dihydrochloride, 4ʹ,6-Diamidino-2-phenylindole, 2HCl

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About This Item

Formula empirica (notazione di Hill):
C16H15N5 · 2HCl
Numero CAS:
28718-90-3
Peso molecolare:
350.25
Numero MDL:
MFCD00012681
Codice UNSPSC:
12352200
NACRES:
NA.54

Livello qualitativo

100

Saggio

≥90% (HPLC)

Forma fisica

solid

Produttore/marchio commerciale

Calbiochem®

Condizioni di stoccaggio

OK to freeze
desiccated (hygroscopic)
protect from light

Colore

yellow

Solubilità

water: 5 mg/mL
ethanol: soluble
methanol: soluble

Fluorescenza

λex ~359 nm
λem ~461 nm

Condizioni di spedizione

ambient

Temperatura di conservazione

−20°C

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
FPNZBYLXNYPRLR-UHFFFAOYSA-N

Descrizione generale

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H2O (Cat. No. 508741).
Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.

Azioni biochim/fisiol

Cell permeable: no
Primary Target
Binds preferentially to DNA rich in adenine and thymine
Product does not compete with ATP.
Reversible: yes

Attenzione

Toxicity: Standard Handling (A)

Ricostituzione

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.

Altre note

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H315,H317,H335

Classi di pericolo

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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