Passa al contenuto
Merck
Tutte le immagini(1)

Documenti

05-1533

Sigma-Aldrich

Anti-Gemin3 Antibody, clone 12H12

clone 12H12, from mouse

Sinonimo/i:

Component of gems 3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 20, DEAD box protein 20, DEAD box protein DP 103, DEAD-box protein DP103, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD, SMN-interacting protein

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

12H12, monoclonal

Reattività contro le specie

human

tecniche

western blot: suitable

N° accesso NCBI

N° accesso UniProt

Condizioni di spedizione

wet ice

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... DDX20(11218)

Descrizione generale

Gemin3 is a member of the DEAD box protein family. The members of this family are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). Some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Gemin 3 is a component of the survival of motor neurons (SMN) complex and codes for a DEAD box protein with an ATPase activity The SMN complex is essential for the biogenesis of spliceosomal small nuclear ribonucleoproteins and likely functions in the assembly, metabolism, and transport of a diverse number of other ribonucleoproteins. Specifically, the SMN complex assembles 7 Sm proteins (Gemin2-8) into a core structure around a highly conserved sequence of ribonucleic acid (RNA) found in small nuclear RNAs. The complex recognizes specific sequences and structural features of small nuclear RNAs and Sm proteins and assembles small nuclear ribonucleoproteins in a stepwise fashion.

Specificità

This antibody recognizes Gemin-3.

Immunogeno

Epitope: Unknown
Recombinant protein corresponding to human Gemin-3.

Applicazioni

Anti-Gemin3 Antibody, clone 12H12 is a Mouse Monoclonal Antibody for detection of Gemin3 also known as Component of gems 3, DEAD-box protein DP103 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins

Qualità

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Gemin-3 on 10 µg of HeLa cell lysate.

Descrizione del bersaglio

Detects a band of approximately 105 kDa (Calculated molecular weight: 92 kDa)

Stato fisico

Format: Purified
Protein G Purified
Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Risultati analitici

Control
HeLa cell lysate

Altre note

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Motore di ricerca dei prodotti.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Sukhleen Kour et al.
Nature communications, 12(1), 2558-2558 (2021-05-09)
GEMIN5, an RNA-binding protein is essential for assembly of the survival motor neuron (SMN) protein complex and facilitates the formation of small nuclear ribonucleoproteins (snRNPs), the building blocks of spliceosomes. Here, we have identified 30 affected individuals from 22 unrelated
Anne-Sophie Gribling-Burrer et al.
Nucleic acids research, 45(9), 5399-5413 (2017-01-25)
Selenoprotein synthesis requires the co-translational recoding of a UGASec codon. This process involves an RNA structural element, called Selenocysteine Insertion Sequence (SECIS) and the SECIS binding protein 2 (SBP2). Several selenoprotein mRNAs undergo unusual cap hypermethylation by the trimethylguanosine synthase

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.