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L1668

Sigma-Aldrich

Lectine de Phaseolus vulgaris (haricot rouge)

Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture

Synonyme(s) :

Agglutinine de Phaseolus vulgaris, PHA

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5 MG
75.80 CHF

75.80 CHF


Date d'expédition estimée le26 mai 2025



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5 MG
75.80 CHF

About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

75.80 CHF


Date d'expédition estimée le26 mai 2025


Niveau de qualité

Gamme de produits

BioReagent

Forme

lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)

Puissance

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

Technique(s)

cell culture | mammalian: suitable

Couleur

white to off-white

Solubilité

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

Température de stockage

2-8°C

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Application

Lectin from Phaseolus vulgaris (red kidney bean) has been used:
  • for the stimulation of cluster of differentiation (CD4+) T cells[1]
  • for the activation of primary peripheral blood lymphocytes[2]
  • for the stimulation of peripheral blood mononuclear cells[3][4]

Actions biochimiques/physiologiques

La PHA correspond à deux espèces moléculaires : l′érythroagglutinine (PHA-E) qui possède une faible activité mitogénique et une forte activité érythroagglutinine, et la leucoagglutinine (PHA-L) qui possède une forte activité mitogénique et leucoagglutinante, mais une activité érythroagglutinante très faible.
La PHA-E n′est pas spécifique au groupe sanguin, mais l′agglutination peut être inhibée par certains oligosaccharides. La PHA-P est la forme protéique de la PHA avant la séparation et la purification de l′érythroagglutinine et de la leucoagglutinine. La PHA-M est la forme mucoprotéique. Les conjugués sont préparés à partir des lectines purifiées correspondantes.

Conditionnement

Sold on the basis of lectin content

Remarque sur l'analyse

Potency and solubility expressed in mg lectin/ml

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)


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Consulter la Bibliothèque de documents

Nina Thakkar et al.
Antimicrobial agents and chemotherapy, 53(2), 631-638 (2008-12-03)
The present studies were conducted to better define the mechanism of action of polyethylene hexamethylene biguanide (PEHMB) (designated herein as NB325), which was shown in previous studies to inhibit infection by the human immunodeficiency virus type 1 (HIV-1). Fluorescence-activated flow
Cellular RelB interacts with the transactivator Tat and enhance HIV-1 expression
Wang M, et al.
Retrovirology, 15(1), 65-65 (2018)
Neslihan Sinim Kahraman et al.
International journal of stem cells, 14(1), 47-57 (2020-10-31)
This study shows the clinical data of 1-year follow-up of 8 patients with degenerative macular diseases who received suprachoroidal adipose tissue derived mesenchymal stem cell (ADMSC) implantation. This prospective, single-center, phase 1/2 study enrolled 8 eyes of 8 patients with
Frank W King et al.
Stem cells and development, 20(3), 475-484 (2010-07-14)
Pluripotent human embryonic stem cells (hESCs) provide an unprecedented opportunity for the study of human tissue development, and the development of cell-based therapies for human disease. To realize these potential advances, however, methods for monitoring expression of intracellular proteins in
Cody J Warren et al.
PLoS biology, 17(6), e3000304-e3000304 (2019-06-11)
Individuals chronically infected with HIV-1 harbor complex viral populations within their bloodstreams. Recently, it has come to light that when these people infect others, the new infection is typically established by only one or a small number of virions from

Articles

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Questions

1–7 of 7 Questions  
  1. What do the designations -P, -L, -M, and -E represent in the names of PHA products?

    1 answer
    1. Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:

      1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.

      2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.

      PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.

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  2. How should the PHA solution using Phytohemagglutinin PHA-P powder be prepared for cell culture?

    1 answer
    1. The stock solution of this product can be prepared in phosphate buffered saline at pH 6.8, resulting in a clear to slightly hazy solution at a concentration of 5 mg per mL. This stock solution can then be diluted in cell culture medium to a working concentration of 10 micrograms of lectin per mL for use in peripheral blood cell culture.

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  3. What is the shelf life once reconstituted in PBS?

    1 answer
    1. After reconstitution in PBS, this product can be stored at 2-8°C for 2-4 weeks. PHA loses approximately 30% activity after 1 month. Stock solutions of at least 1 mg/mL can be stored in frozen aliquots. Do not dilute to the working concentration until ready to use. Vortex stock solutions prior to use.

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  4. What is the solution stability for Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder?

    1 answer
    1. The solution stability was not evaluated by Sigma-Aldrich; however, references indicate media containing dilute PHA can be stored refrigerated for 2-4 weeks. PHA loses approximately 30% activity after 1 month. Stock solutions can be stored in frozen aliquots.

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  5. How can Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder, be dissolved?

    1 answer
    1. Product No. L1668 is solubility tested using 5 mg per mL of phosphate buffered saline (PBS), pH 6.8; this yields a clear to slightly hazy, colorless to light yellow solution.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  7. What is the mitogenic activity of Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder?

    1 answer
    1. The minimum specification for mitogenic activity of this product is  < or = 10 micrograms lectin/mL of medium. The reported specification is PASS. A specific value is not given.

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