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L8777

Sigma-Aldrich

Lectine de Phytolacca americana (phytolaque)

lyophilized powder, BioReagent, suitable for cell culture

Synonyme(s) :

Mitogène de la phytolaque, PWM

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About This Item

Numéro MDL:
MFCD00161973
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Stérilité

aseptically filled

Niveau de qualité

200

Gamme de produits

BioReagent

Forme

lyophilized powder

Puissance

≤0.3 μg/mL mitogenic activity

Technique(s)

cell culture | mammalian: suitable

Solubilité

PBS: 1 mg/mL

Température de stockage

2-8°C

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Catégories apparentées

Description générale

Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.

Application

Lectin from Phytolacca americana (pokeweed) is used for proliferation Assay. (Cellular proliferative responses were measured by the incorporation of 3H thymidine in newly synthesized DNA, using a conventional assay).

Actions biochimiques/physiologiques

Lectins are carbohydrate-binding proteins and are highly specific for sugar moieties. Pokeweed lectin (PL) is a lectin specific for N-acetylglucosamine-containing saccharides that stimulate peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins are isolated from the roots of pokeweed (Phytolacca americana). They contain homologous domains but have different molecular sizes and biological properties.
Le PWM (mitogène de la phytolaque) est extrait des racines de phytolaque. Cette protéine a des propriétés mitogènes, d′hémagglutination et d′agglutination des leucocytes. Le PWM n′est pas spécifique à un groupe sanguin, mais il présente une affinité pour les oligomères du type N-acétyl-β-D-glucosamine.

Qualité

Partially purified TCA precipitate.
Essentially salt-free.

Remarque sur l'analyse

L′activité mitogène a été déterminée par incorporation de BrdU dans les cellules HeLa en culture.

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H317,H334

Conseils de prudence

P261 - P280 - P342 + P311

Classification des risques

Resp. Sens. 1 - Skin Sens. 1

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Tomomi Fujii et al.
Acta crystallographica. Section D, Biological crystallography, 60(Pt 4), 665-673 (2004-03-25)
Pokeweed lectin (PL), a lectin specific for N-acetylglucosamine-containing saccharides, stimulates peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins have been isolated from the roots of pokeweed (Phytolacca americana) and shown to contain homologous
Caroline Leeth et al.
Animals : an open access journal from MDPI, 11(10) (2021-10-24)
Immunological studies in the horse are frequently hampered by lack of environmental control, complicated study design, and ethical concerns when performing high risk studies. The purpose of the current study was to investigate the utility of a xenograft model for
Kurt Hanevik et al.
The Journal of infectious diseases, 204(11), 1779-1786 (2011-10-13)
Clinical and epidemiological studies have suggested the development of acquired immunity in individuals previously infected with Giardia lamblia. However, there are no data on the long-term cellular immunity and genotype cross-reactivity. An outbreak of assemblage B giardiasis in a nonendemic
Samira Rezaei et al.
Scientific reports, 10(1), 7471-7471 (2020-05-06)
In the routine commercial karyotype analysis on 5,481 boars, we identified 32 carriers of mosaic reciprocal translocations, half of which were carrying a specific recurrent translocation, mos t(7;9). An additional 7 mosaic translocations were identified through lymphocyte karyotype analysis from
Sharon N Cox et al.
Kidney international, 78(4), 396-407 (2010-05-21)
Immunoglobulin A nephropathy (IgAN) is the most common form of primary glomerulonephritis worldwide. The basic defect lies within the IgA immune system and in peripheral blood leukocytes, rather than local kidney abnormalities. To define the intracellular mechanisms leading to the
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Articles

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

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