E1131
Exonuclease III from Escherichia coli BE25 /psGR3
buffered aqueous glycerol solution
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About This Item
Produits recommandés
Qualité
for molecular biology
Forme
buffered aqueous glycerol solution
Poids mol.
28 kDa
Numéro d'accès UniProt
Température de stockage
−20°C
Informations sur le gène
Escherichia coli K12 ... xthA(946254)
Description générale
A 3′→5′ exonuclease which catalyzes the removal of mononucleotides from the 3′-end of dsDNA. The enzyme also has apurinic and apyrimidinic endonuclease, 3′-DNA phosphatase, and RNase H activities. Activity is strongly dependent on temperature, salt concentration, and the ratio of enzyme to DNA, therefore reaction conditions must be optimized for specific applications.
Application
Suitable for:
- Production of strand specific probes
- Preparation of single stranded templates for Sanger dideoxy sequencing
- Site directed mutagenesis
Composants
Exonuclease III is supplied as a solution in 5 mM potassium phosphate (pH 6.5), 200 mM KCl, 0.05 mM EDTA, 5 mM 2-mercaptoethanol, 200 μg/ml BSA, and 50% glycerol.
Définition de l'unité
One unit releases 1 nmole of acid-soluble nucleotides from sonicated calf thymus DNA in 30 min at 37 °C.
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution
Ribonucléase A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificats d'analyse (COA)
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Ming Luo et al.
Chemical communications (Cambridge, England), 48(59), 7416-7418 (2012-06-21)
An amplified multiplexed DNA detection biosensor has been developed, which combines the unique cleavage function of exonuclease III (Exo III) with the separating ability of magnetic microparticles (MMPs). By using different fluorophores, the multiplexed detection of DNA is demonstrated.
L H Guo et al.
Nucleic acids research, 10(6), 2065-2084 (1982-03-25)
We describe improve enzymatic methods for sequencing method for sequencing DNA. They are based on partial digestion of duplex DNA with exonuclease III to produce DNA molecules with 3' ends shortened to varying lengths, followed by repair synthesis to extend
Peng Hu et al.
Biosensors & bioelectronics, 34(1), 83-87 (2012-03-03)
We report here a graphene oxide (GO)-based fluorescent aptasensor for adenosine detection by employing exonuclease III (Exo III) as a signal amplifying element. In the absence of adenosine, the adenosine aptamers hybridized with the complementary DNA (cDNA), and the Exo
Sai Bi et al.
Chemical communications (Cambridge, England), 48(7), 1018-1020 (2011-12-07)
The network consisting of three kinds of unlabeled stem-loop DNA molecular beacons (MBs) is activated by target DNA in the presence of exonuclease-III (Exo-III), achieving the concept of exonuclease-assisted cascaded recycling amplification (Exo-CRA) for DNA detection with a wide dynamic
Xiaofeng Wei et al.
Chemical communications (Cambridge, England), 48(49), 6184-6186 (2012-05-17)
Based on the specific folic acid-folate receptor (FA-FR) interaction, macromolecular FR can bind with FA-linked DNA-small molecule chimeras, which can prevent enzymolysis by exonuclease III (Exo III), enabling a novel fluorescence biosensor for FR to be developed using quinaldine red
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