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AP0100

Sigma-Aldrich

Alkaline Phosphatase Diethanolamine Activity Kit

sufficient for 100 tests

Synonyme(s) :

ALP Activity Kit

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.26

Utilisation

sufficient for 100 tests

Température de stockage

2-8°C

Description générale

The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate.

Application

Alkaline Phosphatase Diethanolamine Activity Kit has been used:
  • in the detection of alkaline phosphatase (ALP) levels in the blood serum and osteoblasts
  • for monitoring ALP levels during osteoblast differentiation in mouse myoblast C2C12 cells
  • for determining ALP activity in the bone metastasis model of lung cancer
  • for measuring ALP levels in colon tissue homogenates

Adéquation

Suitable for the measurement of alkaline phosphatase activity in a variety of samples

Principe

Alkaline phosphatase hydrolyzes pNPP to p-nitrophenol and inorganic phosphate. During incubation of the alkaline phosphatase sample and substrate at 37 C, the reaction is followed by monitoring the increase in absorbance at 405 nm.

Composants de kit également disponibles séparément

Réf. du produit
Description
FDS

  • Alkaline Phosphatase Diethanolamine Reaction Buffer

  • P4744Phosphatase substrate, powderFDS

  • P6774Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous solution, ≥2,000 DEA units/mg proteinFDS

Pictogrammes

Health hazardCorrosion

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Organes cibles

Kidney,Liver,Blood

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Consulter la Bibliothèque de documents

Jianming Chen et al.
International journal of molecular medicine, 43(3), 1513-1521 (2019-01-11)
MicroRNAs (miRNAs) have emerged as pivotal regulators in various physiological and pathological processes at the post‑transcriptional level, and may serve important roles in osteogenic differentiation. However, their roles and functions are not fully understood. In the present study, miR‑223‑5p was
Triptolide inhibits the function of TNF-alpha in osteoblast differentiation by inhibiting the NF-kappaB signaling pathway
Liu SP, et al.
Experimental and Therapeutic Medicine, 14(3), 2235-2240 (2017)
Xiang Li et al.
Stem cells international, 2019, 2743047-2743047 (2019-11-30)
MicroRNAs (miRNAs) influence stem cell functions, including mobilization, proliferation, and differentiation. miR-150 is abundantly expressed in monocytes. Knockdown of miR-150 promotes bone marrow stem cell migration. The role of miR-150 in adipose-derived stem cells (ADSCs) is unclear. In this study
Local level of TGF-beta1 determines the effectiveness of dexamethasone through regulating the balance of Treg/Th17 cells in TNBS-induced mouse colitis
You P, et al.
Experimental and Therapeutic Medicine, 15(4), 3639-3649 (2018)
Ribusurong Pu et al.
Experimental and therapeutic medicine, 15(1), 977-984 (2018-02-13)
The aim of the present study was to investigate the therapeutic effect of deer antler extract on avascular necrosis of the femoral head (ANFH) induced by steroids, and to confirm that 11β-hydroxysteroid dehydrogenases (11β-HSD) are one of the targets of

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