D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

• Synonyme(s) : DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I
• Numéro CAS: 9003-98-9
• Numéro de classification (Commission des enzymes): 3.1.21.1 (BRENDA, IUBMB)
• Numéro CE : 232-667-0
• Numéro MDL: MFCD00130918
• Code UNSPSC : 12352204
• Nomenclature NACRES : NA.53

Propriétés

• Source biologique: bovine pancreas
• Niveau de qualité: 200
• Qualité: for molecular biology
• Forme: buffered aqueous glycerol solution
• Activité spécifique: 10000 units/mg protein
• Poids mol.: 29.1 kDa
• Concentration: 5—15 mg protein/mL
• Technique(s): DNA extraction: suitable
• Adéquation: suitable for molecular biology
• Numéro d'accès UniProt: P00639
• Application(s): cell analysis
• Activité étrangère: RNase, none detected
• Température de stockage: −20°C
• Informations sur le gène: cow ... DNASE1(282217)

Description

Description générale

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent.[1] In the presence of Mg²⁺, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn²⁺, both strands can be cleaved.

Application

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.[2][3][4]

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Composants

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

Définition de l'unité

One unit will cause a ΔA₂₆₀ of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

Autres remarques

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

Informations sur la sécurité

• Pictogrammes: GHS08
• Mention d'avertissement: Danger
• Mentions de danger: H334
• Conseils de prudence: P261 - P284 - P501
• Classification des risques: Resp. Sens. 1
• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, type N95 (US)