HPLC Analysis of Fusarium Mycotoxins on Ascentis® Express Phenyl-Hexyl
Materialien
Analytische Säule
Produkt-Nr.
Beschreibung
Preisangaben
Ascentis® Express Phenyl-Hexyl, 2,7 μm HPLC-Säule
2.7 μm particle size, L × I.D. 10 cm × 2.1 mmCONDITIONS
column
Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)
mobile phase
[A] water, acetonitrile, 90:10; [B] acetonitrile
gradient
0% B held for 0.5 min; to 15% B in 1 min; held at 15% B for 6 min
flow rate
0.3 mL/min
pressure
192 bar (2780 psi)
column temp.
30 °C
detector
UV, 218 nm
injection
10 μL
sample
5 mg/L ea. in 95:5, water:acetonitrile
Beschreibung
Hinweis zur Analyse
The trichothecene vomitoxins of Fusarium are significant plant pathogens of grain and forage crops that can lead to anorexic effects with livestock. This application demonstrates resolution of deoxynivalenol and three of its analogs.
Rechtliche Hinweise
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany