Direkt zum Inhalt

Lectin coated MgO nanoparticle: its toxicity, antileishmanial activity, and macrophage activation.

Lectin coated MgO nanoparticle: its toxicity, antileishmanial activity, and macrophage activation.

Drug and chemical toxicology (2014-01-08)

Ali Jebali, Seyedhossein Hekmatimoghaddam, Bahram Kazemi, Azra Allaveisie, Alireza Masoudi, Karim Daliri, Najme Sedighi, Javad Ranjbari

ZUSAMMENFASSUNG

The purpose of this research was to evaluate toxicity of uncoated magnesium oxide nanoparticles (MgO NPs), MgO NPs coated with Peanut agglutinin (PNA) lectin, and PNA alone on the promastigotes of Leishmania major (L. major) and macrophages of BALB/c mice. On the other hand, antileishmanial property of uncoated MgO NPs, lectin coated MgO NPs, and PNA lectin alone was evaluated, and also macrophage activation was investigated after treatment with these materials by measurement of nitrite, H2O2, and some interleukins. This study showed that PNA lectin and lectin coated MgO NPs had approximately no toxicity on L. major and macrophages, but some toxic effects were observed for uncoated MgO NPs, especially at concentration of 500 µg/mL. Interestingly, lectin coated MgO NPs had the highest antileishmanial activity and macrophage activation, compared with uncoated MgO NPs and PNA lectin.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

Sigma-Aldrich

Methanol, suitable for HPLC, ≥99.9%

Sigma-Aldrich

2-Propanol, suitable for HPLC, 99.9%

Sigma-Aldrich

2-Propanol, ACS reagent, ≥99.5%

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Wasserstoffperoxid -Lösung, contains inhibitor, 30 wt. % in H₂O, ACS reagent

Sigma-Aldrich

Methanol, suitable for HPLC, gradient grade, ≥99.9%

Sigma-Aldrich

Wasserstoffperoxid -Lösung, 30 % (w/w) in H₂O, contains stabilizer

Sigma-Aldrich

Methanol, HPLC Plus, ≥99.9%

Sigma-Aldrich

2-Propanol, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%

Sigma-Aldrich

Natriumnitrit, ACS reagent, ≥97.0%

Sigma-Aldrich

Natriumnitrit, ReagentPlus^®, ≥99.0%

Sigma-Aldrich

Wasserstoffperoxid -Lösung, 50 wt. % in H₂O, stabilized

Sigma-Aldrich

Isopropylalkohol, meets USP testing specifications

Sigma-Aldrich

Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%

Sigma-Aldrich

Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)

Sigma-Aldrich

2-Propanol, Laboratory Reagent, ≥99.5%

Sigma-Aldrich

2-Propanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)

Sigma-Aldrich

Methanol, Laboratory Reagent, ≥99.6%

Sigma-Aldrich

Wasserstoffperoxid-Lösung, 30% (w/w), puriss. p.a., reag. ISO, reag. Ph. Eur.

Sigma-Aldrich

Methanol, BioReagent, ≥99.93%

Sigma-Aldrich

Methanol, Absolute - Acetone free

Sigma-Aldrich

Methanol, ACS spectrophotometric grade, ≥99.9%

Sigma-Aldrich

Isopropylalkohol, ≥99.7%, FCC, FG

Sigma-Aldrich

2-Propanol, for molecular biology, BioReagent, ≥99.5%

Sigma-Aldrich

2-Propanol, puriss. p.a., ACS reagent, ≥99.8% (GC)

Sigma-Aldrich

Methanol, anhydrous, 99.8%

Sigma-Aldrich

2-Propanol, BioUltra, for molecular biology, ≥99.5% (GC)

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Wasserstoffperoxid -Lösung, contains ~200 ppm acetanilide as stabilizer, 3 wt. % in H₂O

Sigma-Aldrich

2-Propanol, anhydrous, 99.5%