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Wichtige Dokumente
47868
D-Biotin
analytical standard
Synonym(e):
Biotin, D-Biotin, Bios II, Coenzym R, Vitamin B7, Vitamin H
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About This Item
Empirische Formel (Hill-System):
C10H16N2O3S
CAS-Nummer:
Molekulargewicht:
244.31
Beilstein:
86838
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12164500
PubChem Substanz-ID:
Empfohlene Produkte
Qualität
analytical standard
Leistungsmerkmale
standard type water soluble vitamin
Verpackung
ampule of 100 mg
Methode(n)
HPLC: suitable
gas chromatography (GC): suitable
mp (Schmelzpunkt)
231-233 °C (lit.)
Anwendung(en)
pharmaceutical (small molecule)
vitamins, nutraceuticals, and natural products
Format
neat
Lagertemp.
2-8°C
SMILES String
[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2
InChI
1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1
InChIKey
YBJHBAHKTGYVGT-ZKWXMUAHSA-N
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Allgemeine Beschreibung
D-biotin is water soluble vitamin, which belongs to the B-complex group of vitamins.[1] It generally serves as a covalently-bound coenzyme for several carboxylases[1]. It is an essential micronutrient required for normal cell functions and can be obtained both dietary sources as well as gut microbiota.[2]
Anwendung
D-Biotin may be used as an analytical reference standard for the quantification of the analyte in food samples using high-performance liquid chromatography (HPLC)/avidin-binding assay.[3]
Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Lipopolysaccharide inhibits colonic biotin uptake via interference with membrane expression of its transporter: a role for a casein kinase 2-mediated pathway
Lakhan R and Said.MS
American Journal of Physiology. Cell Physiology, 312, C376-C384 (2017)
Pharmacological Effects of Biotin in Animals
L R-N and C F-M
Mini-Reviews in Medicinal Chemistry, 17, 529-529 (2017)
Determination of the biotin content of select foods using accurate and sensitive HPLC/avidin binding
Staggs GC, et al.
Journal of food composition and analysis, 17(6), 767-776 (2004)
Tazu Aoki et al.
Neuron, 78(5), 881-894 (2013-05-21)
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What is the Department of Transportation shipping information for this product?
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How do I solubilize Product 47868, D-Biotin?
1 answer-
Per the chemicals encyclopedia published by the Royal Society of Chemistry, 13th ed, biotin is readily soluble in hot water or dilute alkali; it is insoluble in other common organic solvents. In our labs, the basic solutions may be Trizma base, 2M ammonium hydroxide, or 1N sodium hydroxide. You can put the biotin into water, and titrate it with base until it goes into solution.
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Is Product 47868, D-Biotin, suitable for cell culture?
1 answer-
Product 47868 D-Biotin, is not cell culture tested, and you should not consider it for cell culture use. The Supelco biotin is a chemical standard to be used in chromatographic methods. For cell culture, consider either the animal cell culture tested biotin (Product No. B4639), or the plant cell culture tested biotin (Product No. B3399).
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What column do you recommend for the analysis of water soluble vitamins, including Biotin?
1 answer-
The Discovery C18 and Ascentis RP-Amide HPLC columns have been used for the separation of ten water soluble vitamins: Ascorbic acid, Biotin, Cyanocobalamin, Folic acid, Niacin, Niacinamide, Pantothenic acid, Pyridoxine, Riboflavin, and Thiamine. Contact Supelco Technical Service for details -- [email protected]
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