ZRB1397

Anti-α-amylase Antibody, clone 1G14 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(e): 1,4-alpha-D-glucan glucanohydrolase 1, Alpha-amylase 1A, EC:3.2.1.1, Salivary alpha-amylase
• UNSPSC-Code: 12352203
• NACRES: NA.46

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Rekombinant: expressed in HEK 293 cells
• Konjugat: unconjugated
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: 1G14, recombinant monoclonal
• Beschreibung: recombinant, expressed in HEK 293 cells
• Produktlinie: ZooMAb^® learn more
• Form: lyophilized
• Mol-Gew.: calculated mol wt 57.77 kDa; observed mol wt ~52 kDa
• Aufgereinigt durch: using Protein A
• Speziesreaktivität: human
• Verpackung: antibody small pack of 25 μL
• Grünere Alternativprodukt-Eigenschaften: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• Erweiterte Validierung: recombinant expression; Learn more about Antibody Enhanced Validation
• Methode(n): affinity binding assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• Isotyp: IgG
• Epitopsequenz: C-terminal half
• Protein-ID-Hinterlegungsnummer: NP_004029
• UniProt-Hinterlegungsnummer: P0DUB6
• Grünere Alternativprodukt-Kategorie: Aligned
• Versandbedingung: ambient
• Lagertemp.: 2-8°C
• Angaben zum Gen: human ... AMY1A(276)

Beschreibung

Allgemeine Beschreibung

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Spezifität

Clone 1G14 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects -amylase. It targets an epitope within 14 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids from the C-terminal half of human -amylase.

Anwendung

Quality Control Testing

Evaluated by Western Blotting in Human Pancreas tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected -amylase in Human pancreas tissue lysate.

Tested Applications

Flow Cytometry Analysis: 1 µg from a representative lot detected -amylase in MCF-7 cells.

Affinity Binding Assay: A representative lot of this antibody bound -amylase peptide with a KD of 7.5 x 10-9 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected -amylase in MCF-7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Zielbeschreibung

Alpha-amylase 1A (UniProt: P0DUB6; also known as EC:3.2.1.1, 1,4-alpha-D-glucan glucanohydrolase 1, Salivary alpha-amylase) is encoded by the AMY1A (also known as AMY1) gene (Gene ID: 276) in human. α-amylase is a major form of amylase found in mammals. It is a calcium-binding enzyme that catalyzes the hydrolysis of α-1,4-glucan linkages to produce oligosaccharides. It is synthesized with a signal peptide (aa 1-15) that is subsequently cleaved off to produce the mature enzyme. It is primarily produced in the salivary glands and pancreas. Three distinct genes (AMY1A, AMY1B and AMY1C), located in a gene cluster on 1p21 are reported to encode proteins sharing the same peptide sequence. Elevated levels of amylase activity in serum are characteristics of acute pancreatitis or salivary lesions. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ramasubbu, N., et al. (2003). J. Mol. Biol. 325(5); 1061-1076).

Physikalische Form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Rekonstituierung

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Rechtliche Hinweise

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable