W3516

Anti-WSTF (C-Terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-BAZ1B, Anti-Bromdomain Adjacent to Zn Finger Domain 1B, Anti-Williams Syndrome Transcription Factor, Anti-Williams-Beuren Syndrome Chromosome Region 9
• MDL-Nummer: MFCD08705535
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Konjugat: unconjugated
• Antikörperform: affinity isolated antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Form: buffered aqueous solution
• Mol-Gew.: antigen 175 kDa
• Speziesreaktivität: human, mouse
• Methode(n): immunoprecipitation (IP): 5-10 μg using from lysates of HEK293-T cells; indirect immunofluorescence: 10-20 μg/mL using paraformaldehyde-fixed NIH3T3 cells; microarray: suitable; western blot: 0.5-1 μg/mL using extracts of MCF-7 breast cancer cell line
• UniProt-Hinterlegungsnummer: Q9UIG0
• Versandbedingung: dry ice
• Lagertemp.: −20°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... BAZ1B(9031) ; mouse ... Baz1b(22385) ; rat ... Baz1b(368002)

Beschreibung

Allgemeine Beschreibung

Williams syndrome transcription factor (WSTF) belongs to the WAL/BAZ/WAC protein family, encoded by the bromodomain adjacent to zinc finger domain 1B (BAZ1B) gene. It is a non-secretory protein, which consists of a C-terminal bromodomain adjacent to a PHD (plant homeodomain) finger.

Immunogen

synthetic peptide corresponding to amino acids 1467-1483 of human WSTF, conjugated to KLH via an N-terminal added cysteine residue. The corresponding rat and mouse sequence differ from the immunizing sequence by three amino acids.

Anwendung

Anti-WSTF (C-Terminal) antibody produced in rabbit has been used in western blotting and immunofluorescence.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem./physiol. Wirkung

Williams syndrome transcription factor (WSTF) is implicated in transcription, replication, chromatin remodeling and DNA damage response. It acts as a tyrosine protein kinase. Mutations in this gene are associated with Williams-Beuren syndrome. WSTF might function as an activator of gene expression of cytochrome P450 family 21 subfamily A member 2 (CYP21A2) and CYP19A1.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)