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T7024

Sigma-Aldrich

N,N,N′,N′-Tetramethylethylendiamin

BioReagent, for molecular biology, ≥99% (GC)

Synonym(e):

1,2-Bis(dimethylamino)ethan, TEMED, TMEDA

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About This Item

Lineare Formel:
(CH3)2NCH2CH2N(CH3)2
CAS-Nummer:
110-18-9
Molekulargewicht:
116.20
Beilstein:
1732991
EG-Nummer:
203-744-6
MDL-Nummer:
MFCD00008335
UNSPSC-Code:
41105319
PubChem Substanz-ID:
24900242
NACRES:
NA.31

Qualität

for molecular biology

Produktlinie

BioReagent

Assay

≥99% (GC)

Expl.-Gr.

9.08 %

Brechungsindex

n20/D 1.4179 (lit.)

bp

120-122 °C (lit.)

mp (Schmelzpunkt)

−55 °C (lit.)

Dichte

0.775 g/mL at 20 °C (lit.)

Fremdaktivität

DNase, RNase, protease, none detected

SMILES String

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

InChIKey

KWYHDKDOAIKMQN-UHFFFAOYSA-N

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Anwendung

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.,,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied. TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED. TEMED has been used in the HPLC of small organic molecules.

Menge

Suggested use is 35 μl for 50 ml acrylamide gel solution.

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Lagerklassenschlüssel

3 - Flammable liquids

WGK

WGK 1

Flammpunkt (°F)

61.7 °F - closed cup

Flammpunkt (°C)

16.5 °C - closed cup

Persönliche Schutzausrüstung

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Andrews, A. T.
Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications, 21-24 (1986)
R Barberi et al.
Journal of chromatography. B, Biomedical applications, 683(1), 3-13 (1996-08-09)
Atomic force microscopy (AFM) has been used to probe the surface of a capillary after coating with "soft" polymers, notably polyacrylamides. The aim was the investigation of the efficiency of coverage of the silica surface, so as to reduce or
A V Vasiliskov et al.
BioTechniques, 27(3), 592-594 (1999-09-18)
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules
Sambrook, J. and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 5-5 (2001)
Arvind Chandrasekaran et al.
Lab on a chip, 19(17), 2786-2798 (2019-07-25)
To study respiratory diseases, in vitro airway epithelial models are commonly implemented by culturing airway cells on a porous surface at an air-liquid interface (ALI). However, these surfaces are often supraphysiologically stiff, which is known to affect the organization, maturation
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